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Product Info Summary
| SKU: | A07909-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-MAP4K1/HPK1 Antibody Picoband®
SKU/Catalog Number
A07909-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-MAP4K1/HPK1 Antibody Picoband® catalog # A07909-2. Tested in WB applications. This antibody reacts with Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MAP4K1/HPK1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07909-2)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of rat MAP4K1/HPK1, which shares 88.2% and 100% amino acid (aa) sequence identity with human and mouse MAP4K1/HPK1, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A07909-2 is reactive to Map4k1 in Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
97 kDa
Calculated molecular weight
49229 MW
Background of MAP4K1
Mitogen-activated protein kinase kinase kinase kinase 1 is a protein kinase that in humans is encoded by the MAP4K1 gene. It is also known as HPK1 (Hematopoietic Progenitor Kinase 1). The protein has been shown to play a role in JNK activation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07909-2 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Rat
Positive Control
WB: rat thymus tissue, rat NRK whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of MAP4K1/HPK1 using anti-MAP4K1/HPK1 antibody (A07909-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat thymus tissue lysates,
Lane 2: rat NRK whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAP4K1/HPK1 antigen affinity purified polyclonal antibody (Catalog # A07909-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAP4K1/HPK1 at approximately 97KD. The expected band size for MAP4K1/HPK1 is at 97KD.
Protein Target Info & Infographic
Gene/Protein Information For Map4k1 (Source: Uniprot.org, NCBI)
Gene Name
Map4k1
Full Name
Mitogen-activated protein kinase kinase kinase kinase 1
Weight
49229 MW
Superfamily
protein kinase superfamily
Alternative Names
Fascin-2; Retinal fascin; FSCN2 MAP4K1 HPK1 mitogen-activated protein kinase kinase kinase kinase 1 mitogen-activated protein kinase kinase kinase kinase 1|MAPK/ERK kinase kinase kinase 1|MEK kinase kinase 1|MEKKK 1|hematopoietic progenitor kinase 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Map4k1, check out the Map4k1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Map4k1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MAP4K1/HPK1 Antibody Picoband® (A07909-2)
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