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Product Info Summary
| SKU: | A07599-1 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC-P, WB |
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Product info
Product Name
Anti-MAP3K9 Antibody (N-term)
SKU/Catalog Number
A07599-1
Size
80 µl
Description
Boster Bio Anti-MAP3K9 Antibody (N-term) (Catalog # A07599-1). Tested in WB, IHC-P application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-MAP3K9 Antibody (N-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07599-1)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This MAP3K9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 159-189 amino acids from the N-terminal region of human MAP3K9.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A07599-1 is reactive to MAP3K9 in Human
Reconstitution
Calculated molecular weight
121895 Da
Background of MAP3K9
Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The STE group (homologs of yeast Sterile 7, 11, 20 kinases) consists of 50 kinases related to the mitogen-activated protein kinase (MAPK) cascade families (Ste7/MAP2K, Ste11/MAP3K, and Ste20/MAP4K). MAP kinase cascades, consisting of a MAPK and one or more upstream regulatory kinases (MAPKKs) have been best characterized in the yeast pheromone response pathway. Pheromones bind to Ste cell surface receptors and activate yeast MAPK pathway.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07599-1 is guaranteed for IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
IHC-P: 1:50-1:100
Validation Images & Assay Conditions
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MAP3K9 Antibody (N-term) western blot analysis in SW480 cell line lysates (35ug/lane). This demonstrates the MAP3K9 antibody detected the MAP3K9 protein (arrow).
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Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Protein Target Info & Infographic
Gene/Protein Information For MAP3K9 (Source: Uniprot.org, NCBI)
Gene Name
MAP3K9
Full Name
Mitogen-activated protein kinase kinase kinase 9
Weight
121895 Da
Superfamily
protein kinase superfamily
Alternative Names
Mitogen-activated protein kinase kinase kinase 9, Mixed lineage kinase 1, MAP3K9, MLK1, PRKE1 MAP3K9 MEKK9, MLK1, PRKE1 mitogen-activated protein kinase kinase kinase 9 mitogen-activated protein kinase kinase kinase 9|mixed lineage kinase 1 (tyr and ser/thr specificity)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MAP3K9, check out the MAP3K9 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MAP3K9: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MAP3K9 Antibody (N-term) (A07599-1)
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