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Product Info Summary
| SKU: | A04842 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IHC-P, IF (paraffin) |
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Product info
Product Name
Anti-MAP3K4/Mekk4 Antibody
SKU/Catalog Number
A04842
Size
100ul
Form
Liquid
Description
Boster Bio Anti-MAP3K4/Mekk4 Antibody (Catalog# A04842). Tested in IHC-P, IF (paraffin), ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MAP3K4/Mekk4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04842)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human MAP3K4. Immunogen sequence location: 1281-1330
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A04842 is reactive to MAP3K4 in Human, Mouse
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
72 kDa
Calculated molecular weight
181685 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04842 is guaranteed for ELISA, IHC-P, IF (paraffin) Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry, 1:100 - 1:300
ELISA, 1:5000
Not yet tested in other applications.
Validation Images & Assay Conditions
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Immunohistochemistry analysis of paraffin-embedded human heart tissue, using MAP3K4 Antibody. The picture on the right is blocked with the synthesized peptide.
Protein Target Info & Infographic
Gene/Protein Information For MAP3K4 (Source: Uniprot.org, NCBI)
Gene Name
MAP3K4
Full Name
Mitogen-activated protein kinase kinase kinase 4
Weight
181685 MW
Superfamily
protein kinase superfamily
Alternative Names
MAP3K4;KIAA0213;MAPKKK4;MEKK4;MTK1;Mitogen-activated protein kinase kinase kinase 4;MAP three kinase 1;MAPK/ERK kinase kinase 4;MEK kinase 4;MEKK 4 MAP3K4 MAPKKK4, MEKK 4, MEKK4, MTK1, PRO0412 mitogen-activated protein kinase kinase kinase 4 mitogen-activated protein kinase kinase kinase 4|MAP three kinase 1|MAP/ERK kinase kinase 4|MAPK/ERK kinase kinase 4|MEK kinase 4|SSK2/SSK22 MAP kinase kinase kinase, yeast, homolog of|dJ473J16.1 (mitogen-activated protein kinase kinase kinase 4)|predicted protein of HQ0412
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MAP3K4, check out the MAP3K4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MAP3K4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MAP3K4/Mekk4 Antibody (A04842)
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Customer Q&As
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13 Customer Q&As for Anti-MAP3K4/Mekk4 Antibody
Question
We are currently using anti-MAP3K4/Mekk4 antibody A04842 for human tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2020-02-18
Answer
The anti-MAP3K4/Mekk4 antibody (A04842) has not been tested for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-02-18
Question
Is this A04842 anti-MAP3K4/Mekk4 antibody reactive to the isotypes of MAP3K4?
Verified Customer
Verified customer
Asked: 2020-01-03
Answer
The immunogen of A04842 anti-MAP3K4/Mekk4 antibody is A peptide derived from human MAP3K4. Immunogen sequence location: 1281-1330. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-01-03
Question
Will anti-MAP3K4/Mekk4 antibody A04842 work for IHC with leukemic t-cell?
Verified Customer
Verified customer
Asked: 2019-10-04
Answer
According to the expression profile of leukemic t-cell, MAP3K4 is highly expressed in leukemic t-cell. So, it is likely that anti-MAP3K4/Mekk4 antibody A04842 will work for IHC with leukemic t-cell.
Boster Scientific Support
Answered: 2019-10-04
Question
Does anti-MAP3K4/Mekk4 antibody A04842 work on goat IHC with cerebellum?
Verified Customer
Verified customer
Asked: 2019-09-03
Answer
Our lab technicians have not validated anti-MAP3K4/Mekk4 antibody A04842 on goat. You can run a BLAST between goat and the immunogen sequence of anti-MAP3K4/Mekk4 antibody A04842 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat cerebellum in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-09-03
Question
My question regarding product A04842, anti-MAP3K4/Mekk4 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-08-29
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04842 anti-MAP3K4/Mekk4 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-08-29
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for leukemic t-cell using anti-MAP3K4/Mekk4 antibody A04842. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-06-12
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-12
Question
Would A04842 anti-MAP3K4/Mekk4 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-05-22
Answer
It shows on the product datasheet, A04842 anti-MAP3K4/Mekk4 antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-05-22
Question
Is a blocking peptide available for product anti-MAP3K4/Mekk4 antibody (A04842)?
C. Johnson
Verified customer
Asked: 2018-01-29
Answer
We do provide the blocking peptide for product anti-MAP3K4/Mekk4 antibody (A04842). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-01-29
Question
See attached the WB image, lot number and protocol we used for leukemic t-cell using anti-MAP3K4/Mekk4 antibody A04842. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2017-08-21
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-08-21
Question
I see that the anti-MAP3K4/Mekk4 antibody A04842 works with IHC, what is the protocol used to produce the result images on the product page?
G. Patel
Verified customer
Asked: 2017-02-15
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-02-15
Question
I was wanting to use your anti-MAP3K4/Mekk4 antibody for IHC for mouse leukemic t-cell on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse leukemic t-cell identification?
N. Zhao
Verified customer
Asked: 2016-07-05
Answer
As indicated on the product datasheet, A04842 anti-MAP3K4/Mekk4 antibody has been validated for IHC on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse leukemic t-cell in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2016-07-05
Question
Is there a BSA free version of anti-MAP3K4/Mekk4 antibody A04842 available?
G. Johnson
Verified customer
Asked: 2015-04-29
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-MAP3K4/Mekk4 antibody A04842 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2015-04-29
Question
My question regards to test anti-MAP3K4/Mekk4 antibody A04842 on mouse leukemic t-cell for research purposes, then I may be interested in using anti-MAP3K4/Mekk4 antibody A04842 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
B. Parker
Verified customer
Asked: 2013-02-22
Answer
The products we sell, including anti-MAP3K4/Mekk4 antibody A04842, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2013-02-22
