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Product Info Summary
| SKU: | PB10066 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-TXNL2/GLRX3 Antibody Picoband®
View all Glutaredoxin 3/GLRX3 Antibodies
SKU/Catalog Number
PB10066
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-TXNL2/GLRX3 Antibody Picoband® catalog # PB10066. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TXNL2/GLRX3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB10066)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human TXNL2 recombinant protein (Position: L89-S177). Human TXNL2 shares 93.3% and 94.4% amino acid (aa) sequence identity with mouse and rat TXNL2, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB10066 is reactive to GLRX3 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
37 kDa
Calculated molecular weight
37432 MW
Background of Glutaredoxin 3/GLRX3
Glutaredoxin-3 is a protein that in humans is encoded by the GLRX3 gene. This gene encodes a member of the glutaredoxin family. Glutaredoxins are oxidoreductase enzymes that reduce a variety of substrates using glutathione as a cofactor. The encoded protein binds to and modulates the function of protein kinase C theta. The encoded protein may also inhibit apoptosis and play a role in cellular growth, and the expression of this gene may be a marker for cancer. Pseudogenes of this gene are located on the short arm of chromosomes 6 and 9. Alternatively spliced transcript variants have been observed for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB10066 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Mouse, Rat, Human
Positive Control
WB: human Jurkat whole cell, rat testis tissue, mouse testis tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of TXNL2 using anti-TXNL2 antibody (PB10066).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
Lane 2: rat testis tissue lysates,
Lane 3: mouse testis tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TXNL2 antigen affinity purified polyclonal antibody (Catalog # PB10066) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TXNL2 at approximately 37 kDa. The expected band size for TXNL2 is at 37 kDa.
Protein Target Info & Infographic
Gene/Protein Information For GLRX3 (Source: Uniprot.org, NCBI)
Gene Name
GLRX3
Full Name
Glutaredoxin-3
Weight
37432 MW
Alternative Names
Glutaredoxin-3;PKC-interacting cousin of thioredoxin;PICOT;PKC-theta-interacting protein;PKCq-interacting protein;Thioredoxin-like protein 2;GLRX3;PICOT, TXNL2;HUSSY-22; GLRX3 GLRX4, GRX3, GRX4, PICOT, TXNL2, TXNL3 glutaredoxin 3 glutaredoxin-3|PKC-interacting cousin of thioredoxin|PKC-theta-interacting protein|PKCq-interacting protein|glutaredoxin 4|testicular tissue protein Li 75|thioredoxin-like protein 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GLRX3, check out the GLRX3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GLRX3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TXNL2/GLRX3 Antibody Picoband® (PB10066)
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No publications found for PB10066
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Customer Q&As
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4 Customer Q&As for Anti-TXNL2/GLRX3 Antibody Picoband®
Question
We are currently using anti-TXNL2/GLRX3 antibody PB10066 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-28
Answer
The anti-TXNL2/GLRX3 antibody (PB10066) has not been tested for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-28
Question
Is this PB10066 anti-TXNL2/GLRX3 antibody reactive to the isotypes of GLRX3?
Verified Customer
Verified customer
Asked: 2017-09-12
Answer
The immunogen of PB10066 anti-TXNL2/GLRX3 antibody is E. coli-derived human TXNL2 recombinant protein (Position: L89-S177). Human TXNL2 shares 93.3% and 94.4% amino acid (aa) sequence identity with mouse and rat TXNL2, respectively. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-09-12
Question
I was wanting to use your anti-TXNL2/GLRX3 antibody for WB for human liver on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human liver identification?
R. Jha
Verified customer
Asked: 2017-03-09
Answer
You can see on the product datasheet, PB10066 anti-TXNL2/GLRX3 antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-03-09
Question
Our lab want to know about to test anti-TXNL2/GLRX3 antibody PB10066 on human liver for research purposes, then I may be interested in using anti-TXNL2/GLRX3 antibody PB10066 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
K. Jha
Verified customer
Asked: 2014-04-11
Answer
The products we sell, including anti-TXNL2/GLRX3 antibody PB10066, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2014-04-11
