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Product Info Summary
| SKU: | A15720-2 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-MANSC1 Antibody Picoband®
SKU/Catalog Number
A15720-2
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-MANSC1 Antibody Picoband® catalog # A15720-2. Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-MANSC1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A15720-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human MANSC1 recombinant protein (Position: L21-N337).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A15720-2 is reactive to MANSC1 in Human
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
62 kDa
Calculated molecular weight
24145 MW
Background of MANSC1
MANSC1 (MANSC domain-containing protein 1), also known as LOH12CR3 (Loss of heterozygosity 12 chromosomal region 3 protein), is a 414 amino acid single-pass membrane protein. Expressed throughout the body, MANSC1 contains one MANSC domain and is encoded by a gene that is located on chromosome 12. Encoding over 1,100 genes within 132 million bases, chromosome 12 makes up about 4.5% of the human genome. A number of skeletal deformities are linked to chromosome 12, including hypochondrogenesis, achondrogenesis and Kniest dysplasia. Chromosome 12 is also home to a homeobox gene cluster which encodes crucial transcription factors for morphogenesis, and the natural killer complex gene cluster encoding C-type lectin proteins which mediate the NK cell response to MHC I interaction.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A15720-2 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human RT4 whole cell, human U251 whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of MANSC1 using anti-MANSC1 antibody (A15720-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human RT4 whole cell lysates,
Lane 2: human U251 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MANSC1 antigen affinity purified polyclonal antibody (Catalog # A15720-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MANSC1 at approximately 62 kDa. The expected band size for MANSC1 is at 62 kDa.
Protein Target Info & Infographic
Gene/Protein Information For MANSC1 (Source: Uniprot.org, NCBI)
Gene Name
MANSC1
Full Name
MANSC domain-containing protein 1
Weight
24145 MW
Alternative Names
Kelch repeat and BTB domain-containing protein 2; BTB and kelch domain-containing protein 1; KBTBD2; BKLHD1; KIAA1489 MANSC1 9130403P13Rik, LOH12CR3 MANSC domain containing 1 MANSC domain-containing protein 1|loss of heterozygosity 12 chromosomal region 3 protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MANSC1, check out the MANSC1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MANSC1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MANSC1 Antibody Picoband® (A15720-2)
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