Anti-MAML2 (Mastermind Like Transcriptional Coactivator 2) Monoclonal Antibody

Product Name

Anti-MAML2 (Mastermind Like Transcriptional Coactivator 2) Monoclonal Antibody

View all MAML2 Antibodies

SKU/Catalog Number

M03679

Size

100ug/vial

Form

Liquid

Description

Boster Bio Anti-MAML2 (Mastermind Like Transcriptional Coactivator 2) Monoclonal Antibody (Catalog # M03679). Tested in Flow Cytometry, IF, WB, IHC applications. This antibody reacts with Human.

Storage & Handling

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Cite This Product

Anti-MAML2 (Mastermind Like Transcriptional Coactivator 2) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03679)

Host

Mouse

Contents

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Clonality

Monoclonal

Clone Number

Clone: MAML2/1302

Isotype

IgG2a, kappa

Immunogen

Recombinant human MAML2 fragment

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Does not cross-react with primate, avian or amphibian GR.

Reactive Species

M03679 is reactive to MAML2 in Human

Reconstitution

Reconstitute with distilled water.

Observed Molecular Weight

68 kDa

Calculated molecular weight

125197 MW

Background of MAML2

MAML2 (mastermind-like protein 2), also known as MAM2, MAM3 or MLLMAML2, is a nuclear speckle protein that acts as a transcriptional co-activator for Notch receptors. The Notch signaling pathway influences cell fate by regulating the ability of precursor cells to properly respond to developmental signals. MAML2 is a member of the mastermind-like family of proteins that are human homologs of the Drosophila melanogaster mastermind protein. Through its N-terminal region, MAML2 interacts with the ankyrin repeats of the Notch proteins Notch 1, Notch 2, Notch 3 and Notch 4. This interaction leads to formation of a DNA-binding complex with the Notch proteins and RBP-J a complex that can then induce HES1 gene expression. While the N-terminal domain of MAML2 is essential for proper Notch binding, the C-terminal domain of MAML2 is essential for transcriptional activation. A chromosomal aberration involving the gene encoding MAML2 is implicated in mucoepidermoid carcinomas, clear cell hidradenomas and benign Warthin tumors.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M03679 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues is enhanced by heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0 for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Validation Images & Assay Conditions

Gene/Protein Information For MAML2 (Source: Uniprot.org, NCBI)

Alternative Names

Mastermind-like protein 2;Mam-2;MAML2;KIAA1819; MAML2 MAM-3, MAM2, MAM3, MLL-MAML2 mastermind like transcriptional coactivator 2 mastermind-like protein 2|mam-2|mastermind-like 2

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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