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Product Info Summary
| SKU: | M02357 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Mad2L2 Monoclonal Antibody
SKU/Catalog Number
M02357
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Mad2L2 Monoclonal Antibody catalog # M02357. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Mad2L2 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02357)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
ADFO-13
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Mad2L2
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M02357 is reactive to MAD2L2 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
100 kDa
Calculated molecular weight
101549 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02357 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:1000
IHC 1:100-1:500
ICC/IF 1:50-1:200
IP 1:30
FC 1:40
Positive Control
WB: Hela cell
Validation Images & Assay Conditions
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Western blot analysis of Mad2L2 expression in Hela cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For MAD2L2 (Source: Uniprot.org, NCBI)
Gene Name
MAD2L2
Full Name
Mitotic spindle assembly checkpoint protein MAD2B
Weight
101549 MW
Alternative Names
Phosphatidylinositol 3-kinase catalytic subunit type 3;PI3-kinase type 3;PI3K type 3;PtdIns-3-kinase type 3;2.7.1.137;Phosphatidylinositol 3-kinase p100 subunit;Phosphoinositide-3-kinase class 3;hVps34;PIK3C3;VPS34; MAD2L2 FANCV, MAD2B, POLZ2, REV7 mitotic arrest deficient 2 like 2 mitotic spindle assembly checkpoint protein MAD2B|MAD2 (mitotic arrest deficient, yeast, homolog)-like 2|MAD2 mitotic arrest deficient-like 2|MAD2-like protein 2|REV7 homolog|hREV7|mitotic arrest deficient 2-like protein 2|mitotic arrest deficient homolog-like 2|polymerase (DNA-directed), zeta 2, accessory subunit
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MAD2L2, check out the MAD2L2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MAD2L2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Mad2L2 Monoclonal Antibody (M02357)
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No publications found for M02357
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Customer Q&As
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7 Customer Q&As for Anti-Mad2L2 Monoclonal Antibody
Question
Here is the WB image, lot number and protocol we used for cerebellum embryo using anti-Mad2L2 Monoclonal antibody M02357. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-03-24
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-03-24
Question
Is a blocking peptide available for product anti-Mad2L2 Monoclonal antibody (M02357)?
Verified Customer
Verified customer
Asked: 2020-01-30
Answer
We do provide the blocking peptide for product anti-Mad2L2 Monoclonal antibody (M02357). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-01-30
Question
Will anti-Mad2L2 Monoclonal antibody M02357 work on goat WB with testis?
Verified Customer
Verified customer
Asked: 2020-01-20
Answer
Our lab technicians have not tested anti-Mad2L2 Monoclonal antibody M02357 on goat. You can run a BLAST between goat and the immunogen sequence of anti-Mad2L2 Monoclonal antibody M02357 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat testis in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-01-20
Question
I see that the anti-Mad2L2 Monoclonal antibody M02357 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-01-10
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-01-10
Question
What sample was tested in WB and IHC applications with M02357?
Verified customer
Asked: 2020-01-09
Answer
Western blot analysis of Mad2L2 expression in Hela cell lysate.
Boster Scientific Support
Answered: 2020-01-09
Question
Is this M02357 anti-Mad2L2 Monoclonal antibody reactive to the isotypes of MAD2L2?
Verified Customer
Verified customer
Asked: 2019-11-13
Answer
The immunogen of M02357 anti-Mad2L2 Monoclonal antibody is A synthesized peptide derived from human Mad2L2. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-11-13
Question
We are currently using anti-Mad2L2 Monoclonal antibody M02357 for mouse tissue, and we are satisfied with the ICC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-09
Answer
The anti-Mad2L2 Monoclonal antibody (M02357) has not been validated for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-09
