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Product Info Summary
| SKU: | A08850 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-LIM domain only protein 3 LMO3 Antibody
View all LIM domain only 3 Antibodies
SKU/Catalog Number
A08850
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-LIM domain only protein 3 LMO3 Antibody (Catalog # A08850). Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-LIM domain only protein 3 LMO3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08850)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human LMO3.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A08850 is reactive to LMO3 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
16594 MW
Background of LIM domain only 3
The protein encoded by this gene belongs to the rhombotin family of cysteine-rich LIM domain oncogenes. This gene is predominantly expressed in the brain. Related family members, LMO1 and LMO2 on chromosome 11, have been reported to be involved in chromosomal translocations in T-cell leukemia. Many alternatively spliced transcript variants have been found for this gene.
Ota T., Nat. Genet. 36:40-45(2004).
The MGC Project Team, Genome Res. 14:2121-2127(2004).
Foroni L., J. Mol. Biol. 226:747-761(1992).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A08850 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
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Western blot analysis of extracts from COS-7 cells, using LMO3 antibody A08850.
Protein Target Info & Infographic
Gene/Protein Information For LMO3 (Source: Uniprot.org, NCBI)
Gene Name
LMO3
Full Name
LIM domain only protein 3
Weight
16594 MW
Alternative Names
LIM domain only protein 3;LMO-3;Neuronal-specific transcription factor DAT1;Rhombotin-3;LMO3;RBTN3, RBTNL2, RHOM3; LMO3 RBTN3, RBTNL2, RHOM3, Rhom-3 LIM domain only 3 LIM domain only protein 3|LIM domain only 3 (rhombotin-like 2)|neuronal-specific transcription factor DAT1|rhombotin-3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on LMO3, check out the LMO3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for LMO3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-LIM domain only protein 3 LMO3 Antibody (A08850)
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No publications found for A08850
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-LIM domain only protein 3 LMO3 Antibody
Question
I was wanting to use to test anti-LMO3 antibody A08850 on mouse caudate nucleus for research purposes, then I may be interested in using anti-LMO3 antibody A08850 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-01-22
Answer
The products we sell, including anti-LMO3 antibody A08850, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-01-22
Question
Do you have a BSA free version of anti-LMO3 antibody A08850 available?
Verified Customer
Verified customer
Asked: 2020-01-10
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-LMO3 antibody A08850 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-01-10
Question
Is this A08850 anti-LMO3 antibody reactive to the isotypes of LMO3?
Verified Customer
Verified customer
Asked: 2019-12-04
Answer
The immunogen of A08850 anti-LMO3 antibody is Synthesized peptide derived from internal of human LMO3. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-12-04
Question
We are currently using anti-LMO3 antibody A08850 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2019-11-22
Answer
The anti-LMO3 antibody (A08850) has not been tested for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-22
Question
Does anti-LMO3 antibody A08850 work for WB with caudate nucleus?
Verified Customer
Verified customer
Asked: 2019-08-20
Answer
According to the expression profile of caudate nucleus, LMO3 is highly expressed in caudate nucleus. So, it is likely that anti-LMO3 antibody A08850 will work for WB with caudate nucleus.
Boster Scientific Support
Answered: 2019-08-20
Question
I see that the anti-LMO3 antibody A08850 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-01-17
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-01-17
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for caudate nucleus using anti-LMO3 antibody A08850. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-01-15
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-01-15
