Product Info Summary
SKU: | A03079 |
---|---|
Size: | 100ul |
Reactive Species: | Human |
Host: | Rabbit |
Application: | Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-Lysine-specific demethylase 5A KDM5A Antibody
SKU/Catalog Number
A03079
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Lysine-specific demethylase 5A KDM5A Antibody catalog # A03079. Tested in WB,ICC/IF,Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Lysine-specific demethylase 5A KDM5A Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03079)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human ADAM9 protein.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A03079 is reactive to KDM5A in Human
Applications
A03079 is guaranteed for Flow Cytometry, IF, ICC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
192095 MW
Background of Jarid1A
RRM2B/p53-R2, or p53-inducible ribonucleotide reductase small subunit 2-like protein, is a member of a broad superfamily of ferritin-like di-iron-carboxylate proteins. The RRM2B protein is an enzyme that catalyzes the conversion of ribonucleotides to deoxyribonucleotides that are essential for DNA synthesis, and is found in all eukaryotes. RRM2B plays a pivotal role in cell survival by repairing damaged DNA in a p53/TP53-dependent manner. It supplies deoxyribonucleotides for DNA repair in cells arrested at G1 or G2. It contains an iron-tyrosyl free radical center required for catalysis, and forms an active ribonucleotide reductase (RNR) complex with RRM1 which is expressed both in resting and proliferating cells in response to DNA damage. It is a heterotetramer with a large (RRM1) subunit, and interacts with p53/TP53. The interaction with RRM1 occurs in response to DNA damage and results in its translocation from cytoplasm to nucleus. It is widely expressed at a high level in skeletal muscle and at a weak level in thymus, and expressed in epithelial dysplasias and squamous cell carcinoma. Defects in RRM2B are the cause of encephalomyopathic mitochondrial depletion syndrome with renal tubulopathy (EMDSRT). Mitochondrial DNA depletion syndrome (MDS) is a clinically heterogeneous group of disorders characterized by a reduction in mitochondrial DNA (mtDNA) copy number. The encephalomyopathic form with renal tubulopathy is presented with various combinations of hypotonia, tubulopathy, seizures, respiratory distress, diarrhea, and lactic acidosis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500
ICC/IF: 1:50-1:400
FC: 1:50-1:100
Validation Images & Assay Conditions
Click image to see more details
Figure 2. Immunocytochemistry staining of KDM5A using Anti-Lysine-specific demethylase 5A KDM5A Antibody (A03079).
ICC staining KDM5A in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehydeClick image to see more details
Figure 1. Western blotting validation for Anti-Lysine-specific demethylase 5A KDM5A Antibody A03079
Western blot analysis of KDM5A on A549 cell using anti-KDM5A antibody at 1/500 dilution.Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For KDM5A (Source: Uniprot.org, NCBI)
Gene Name
KDM5A
Full Name
Lysine-specific demethylase 5A
Weight
192095 MW
Superfamily
JARID1 histone demethylase family
Alternative Names
EC 1.14.11; EC 1.14.11.-; Histone demethylase JARID1A; JARID1AJumonji, AT rich interactive domain 1A (RBP2-like); Jumonji, AT rich interactive domain 1A (RBBP2-like); Jumonji/ARID domain-containing protein 1A; KDM5A; lysine (K)-specific demethylase 5A; RBBP-2; RBBP2lysine-specific demethylase 5A; RBP2retinoblastoma binding protein 2; Retinoblastoma-binding protein 2jumonji, AT rich interactive domain 1A KDM5A RBBP-2, RBBP2, RBP2 lysine demethylase 5A lysine-specific demethylase 5A|Jumonji, AT rich interactive domain 1A (RBP2-like)|[histone H3]-trimethyl-L-lysine(4) demethylase 5A|histone demethylase JARID1A|jumonji/ARID domain-containing protein 1A|lysine (K)-specific demethylase 5A|retinoblastoma binding protein 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on KDM5A, check out the KDM5A Infographic
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In this infographic, you will see the following information for KDM5A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Lysine-specific demethylase 5A KDM5A Antibody (A03079)
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