Anti-JMJD1A KDM3A Monoclonal Antibody

Product Name

Anti-JMJD1A KDM3A Monoclonal Antibody

View all Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibodies

SKU/Catalog Number

A03994

Size

100μl

Form

Liquid

Description

Boster Bio Anti-JMJD1A KDM3A Monoclonal Antibody catalog # A03994. Tested in ELISA, IF, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-JMJD1A KDM3A Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03994)

Host

Mouse

Contents

Ascitic fluid containing 0.03% sodium azide, 0.5% BSA, 50% glycerol.

Clonality

Monoclonal

Clone Number

2E1

Isotype

1 mg/mL

Immunogen

Purified recombinant fragment of human JMJD1A expressed in E. Coli.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A03994 is reactive to KDM3A in Human

Applications

A03994 is guaranteed for ELISA, IF, WB Boster Guarantee

Observed Molecular Weight

39 kDa

Calculated molecular weight

147341 MW

Background of Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A

Transcriptional repressor which binds neuron-restrictive silencer element (NRSE) and represses neuronal gene transcription in non-neuronal cells. Restricts the expression of neuronal genes by associating with two distinct corepressors, mSin3 and CoREST, which in turn recruit histone deacetylase to the promoters of REST-regulated genes. Mediates repression by recruiting the BHC complex at RE1/NRSE sites which acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier.

Chong J.A.et.al. (1995)Cell 80:949-957
Schoenherr C.J.et.al.(1995)Science 267:1360-1363
Scholl T.et.al.(1996)J. Immunol. 156:1448-1457
Lunyak V.V.et.al. (2002)Science 298:1747-1752

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IF 1:200-1:1000
ELISA 1:10000

Validation Images & Assay Conditions

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Western Blot (WB) analysis using JMJD1A Monoclonal antibody against HeLa (1) and HepG2 (2) cell lysate.

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Immunofluorescence (IF) analysis of HeLa cells using JMJD1A Monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Gene/Protein Information For KDM3A (Source: Uniprot.org, NCBI)

Alternative Names

EC 1.14.11; EC 1.14.11.-; JHDM2A; JHDM2AJMJD1AJHMD2A; JmjC domain-containing histone demethylation protein 2A; JMJD1; JMJD1A; jumonji C domain-containing histone demethylase 2A; jumonji domain containing 1; jumonji domain containing 1A; Jumonji domain-containing protein 1A; KDM3A; KIAA0742; KIAA0742DKFZp686A24246; Lysine (K)specific Demethylase 3A; Lysine (K)-specific Demethylase 3A; lysine-specific demethylase 3A; testis-specific protein A; TSGA; TSGADKFZp686P07111 KDM3A JHDM2A, JHMD2A, JMJD1, JMJD1A, TSGA lysine demethylase 3A lysine-specific demethylase 3A|[histone H3]-dimethyl-L-lysine(9) demethylase 3A|jmjC domain-containing histone demethylation protein 2A|jumonji C domain-containing histone demethylase 2A|jumonji domain-containing protein 1A|lysine (K)-specific demethylase 3A|testis-specific protein A

For more info on KDM3A, check out the KDM3A Infographic

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In this infographic, you will see the following information for KDM3A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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