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Product Info Summary
| SKU: | M04060 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-LYRIC Monoclonal Antibody
SKU/Catalog Number
M04060
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-LYRIC Monoclonal Antibody catalog # M04060. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-LYRIC Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04060)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
ADIF-13
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human LYRIC Downregulates SLC1A2/EAAT2 promoter activity when expressed ectopically. Activates the nuclear factor kappa-B (NF-kappa-B) transcription factor. Promotes anchorage-independent growth of immortalized melanocytes and astrocytes which is a key component in tumor cell expansion.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M04060 is reactive to MTDH in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
37 kDa
Calculated molecular weight
17184 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M04060 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:5000
IHC 1:50-1:200
ICC/IF 1:50-1:200
Positive Control
WB: HeLa cell
Validation Images & Assay Conditions
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Western blot analysis of LYRIC expression in HeLa cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For MTDH (Source: Uniprot.org, NCBI)
Gene Name
MTDH
Full Name
Protein LYRIC
Weight
17184 MW
Alternative Names
Myoglobin;MB; Mtdh|2610103J23Rik, 3D3, 3D3/Ly, 3D3/Lyric, AEG-, AEG-1, AV353288, D8Bwg1112e, Ly, Lyric|metadherin|protein LYRIC|lysine-rich CEACAM1 co-isolated protein|metastasis adhesion protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MTDH, check out the MTDH Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MTDH: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-LYRIC Monoclonal Antibody (M04060)
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No publications found for M04060
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Customer Q&As
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4 Customer Q&As for Anti-LYRIC Monoclonal Antibody
Question
Would M04060 anti-LYRIC Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-10-02
Answer
You can see on the product datasheet, M04060 anti-LYRIC Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-10-02
Question
Would anti-LYRIC Monoclonal antibody M04060 work for WB with leukemic t-cell?
P. Huang
Verified customer
Asked: 2018-12-04
Answer
According to the expression profile of leukemic t-cell, MTDH is highly expressed in leukemic t-cell. So, it is likely that anti-LYRIC Monoclonal antibody M04060 will work for WB with leukemic t-cell.
Boster Scientific Support
Answered: 2018-12-04
Question
I was wanting to use your anti-LYRIC Monoclonal antibody for WB for rat leukemic t-cell on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat leukemic t-cell identification?
W. Krishna
Verified customer
Asked: 2015-08-05
Answer
You can see on the product datasheet, M04060 anti-LYRIC Monoclonal antibody has been validated for IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat leukemic t-cell in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-08-05
Question
Is this M04060 anti-LYRIC Monoclonal antibody reactive to the isotypes of MTDH?
L. Singh
Verified customer
Asked: 2013-03-08
Answer
The immunogen of M04060 anti-LYRIC Monoclonal antibody is A synthesized peptide derived from human LYRIC Downregulates SLC1A2/EAAT2 promoter activity when expressed ectopically. Activates the nuclear factor kappa-B (NF-kappa-B) transcription factor. Promotes anchorage-independent growth of immortalized melanocytes and astrocytes which is a key component in tumor cell expansion. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2013-03-08
