Anti-LRIG1 Antibody Picoband™

Product Name

Anti-LRIG1 Antibody Picoband™

View all Lrig1 Antibodies

SKU/Catalog Number

A04613-2

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-LRIG1 Antibody Picoband™ catalog # A04613-2. Tested in WB, ELISA applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-LRIG1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04613-2)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Immunogen

E.coli-derived human LRIG1 recombinant protein (Position: K79-E906). Human LRIG1 shares 89.8% amino acid (aa) sequence identity with mouse LRIG1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A04613-2 is reactive to Lrig1 in Human

Applications

A04613-2 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

145 kDa

Calculated molecular weight

Background of Lrig1

Leucine-rich repeats and immunoglobulin-like domains protein 1 is a protein that in humans is encoded by the LRIG1 gene. It is mapped to 3p14.1. Leucine-rich repeats and immunoglobulin-like domains protein 1 is a protein that in humans is encoded by the LRIG1 gene. It encodes a transmembrane protein that has been shown to interact with receptor tyrosine kinases of the EGFR-family, MET and RET. This gene encodes a member of the ATP-dependent DNA ligase protein family. The encoded protein functions in DNA replication, recombination, and the base excision repair process. Mutations in this gene that lead to DNA ligase I deficiency result in immunodeficiency and increased sensitivity to DNA-damaging agents. Disruption of this gene may also be associated with a variety of cancers. Alternative splicing results in multiple transcript variants.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human
ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of LRIG1 using anti-LRIG1 antibody (A04613-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LRIG1 antigen affinity purified polyclonal antibody (Catalog # A04613-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LRIG1 at approximately 145 kDa. The expected band size for LRIG1 is at 119 kDa.

Gene/Protein Information For Lrig1 (Source: Uniprot.org, NCBI)

Alternative Names

D6Bwg0781e; Img; LIG1; LIG-1; LRIG1 Lrig1|D6Bwg0781e, Im, Img, LIG, LIG-1|leucine-rich repeats and immunoglobulin-like domains 1|leucine-rich repeats and immunoglobulin-like domains protein 1|integral membrane glycoprotein

For more info on Lrig1, check out the Lrig1 Infographic

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In this infographic, you will see the following information for Lrig1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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