Click image to see more details
Product Info Summary
| SKU: | PA2040 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Lipoma-preferred partner LPP Antibody Picoband®
SKU/Catalog Number
PA2040
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Lipoma-preferred partner LPP Antibody catalog # PA2040. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Lipoma-preferred partner LPP Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2040)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human LPP.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2040 is reactive to LPP in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
80 kDa
Calculated molecular weight
65746 MW
Background of LPP
LPP (Lim Domain-Containing Preferred Translocation Partner in Lipoma) is a protein that in humans is encoded by the LPP gene. Petit et al. (1996) commented that the LPP-encoded protein should be classified as a novel member of the group 3 proteins of the LIM protein gene family. By partial cDNA cloning, Petit et al. (1996) established features of the genetic organization of LPP. The gene was found to span a genomic region of over 400 kb. By FISH and Southern blot analyses, Daheron et al. (2001) identified a rearrangement in the mixed lineage leukemia gene due to a novel t (3;11) (q28;q23) translocation in a patient who developed acute myeloid leukemia of the M5 type 3 years after treatment for a follicular lymphoma.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA2040 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: human A549 whole cell, human HepG2 whole cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of LPP using anti-LPP antibody (PA2040).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A549 whole cell lysates,
Lane 2: human HepG2 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LPP antigen affinity purified polyclonal antibody (Catalog # PA2040) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LPP at approximately 80 kDa. The expected band size for LPP is at 66 kDa.
Protein Target Info & Infographic
Gene/Protein Information For LPP (Source: Uniprot.org, NCBI)
Gene Name
LPP
Full Name
Lipoma-preferred partner
Weight
65746 MW
Superfamily
zyxin/ajuba family
Alternative Names
Lipoma-preferred partner;LIM domain-containing preferred translocation partner in lipoma;LPP; LPP LIM domain containing preferred translocation partner in lipoma lipoma-preferred partner|LIM protein|lipoma preferred partner
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on LPP, check out the LPP Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for LPP: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Lipoma-preferred partner LPP Antibody Picoband® (PA2040)
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No publications found for PA2040
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-Lipoma-preferred partner LPP Antibody Picoband®
Question
I see that the anti-LPP antibody PA2040 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-11-28
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-11-28
Question
Is a blocking peptide available for product anti-LPP antibody (PA2040)?
Verified Customer
Verified customer
Asked: 2019-09-19
Answer
We do provide the blocking peptide for product anti-LPP antibody (PA2040). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-09-19
Question
We are currently using anti-LPP antibody PA2040 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2019-08-28
Answer
The anti-LPP antibody (PA2040) has not been tested for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-08-28
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for saphenous vein using anti-LPP antibody PA2040. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-02-28
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-02-28
