Lipoma-preferred partner Antibodies

Lipoma-preferred partner (LPP)

May play a structural role at sites of cell adhesion in maintaining cell shape and motility. In addition to these structural functions, it might also be implicated in signaling events and activation of gene transcription.

May be involved in signal transduction from cell adhesion sites to the nucleus allowing successful integration of signals arising from soluble factors and cell-cell adhesion sites. Also suggested to serve as a scaffold protein on which distinct protein complexes are assembled in the cytoplasm and in the nucleus.

Gene Information

Human
Gene Name:	LPP
Uniprot:	Q93052
Entrez:	4026

Family

Belongs to:
zyxin/ajuba family

Alternative Names

DKFZp779O0231; FLJ30652; FLJ41512; LIM domain containing preferred translocation partner in lipoma; LIM domain-containing preferred translocation partner in lipoma; LIM protein; lipoma-preferred partner; LPP

Molecular Weight

Mass (kDA):
65.746 kDA

Genomic Context

Human
Location:	3q27.3-q28
Sequence:	3; NC_000003.12 (188152152..188890671)

Tissue Specificity

Expressed in a wide variety of tissues but no or very low expression in brain and peripheral leukocytes.

Subcellular Localizations

Nucleus. Cytoplasm. Cell junction. Cell membrane. Found in the nucleus, in the cytoplasm and at cell adhesion sites. Shuttles between the cytoplasm and the nucleus. It has been found in sites of cell adhesion such as cell-to-cell contact and focal adhesion which are membrane attachment sites of cells to the extracellular matrix. Mainly nuclear when fused with HMGA2/HMGIC and KMT2A/MLL1.

Diseases

• Urinary Incontinence
• Urinary Stress Incontinence
• Urethral Diseases
• Lipoma
• Neoplasms
• Tissue Adhesions
• Depressive Disorder
• Anxiety Disorders
• Pathological Dilatation
• Lichen Disease
• Neurogenic Urinary Bladder
• Salmonella Infections

• Malignant Neoplasms
• Lichen Planus
• Inflammation
• Pain
• Chromosomal Translocation
• Diabetes Mellitus
• Autoimmune Reaction

Interacting Proteins

• LASP1

Pathways

• Localization
• Secretion
• Pathogenesis
• Cell Migration
• Transport
• Synaptic Transmission
• Cell Adhesion
• Reflex
• Immune Response
• Virulence
• Cell Growth
• Lipid Modification
• Translation

• Regeneration
• Cell Death
• Cell Activation
• Cell Division
• Angiogenesis
• Cell Motility
• Cognition

PTMs

• Cleavage
• Dephosphorylation
• Phosphorylation
• Oxidation

• Acylation
• Methylation
• Nitration
• Biotinylation

• Phosphorothioation

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/LPP

Best Uses Of The LPP Marker From Boster Bio

The LPP marker is among the most frequently used proteins in many biological assays. It reacts with different antibodies made against lipoma's preferred partners. Boster Bio has produced monoclonal and polyclonal antibodies against the protein in various animal models. The laboratory has shown that the LPP protein has an essential structural role at the cell adhesion sites. It could also be involved in the process of signaling or gene transcription.

Lipoma-preferred partner antibodies

Lipoma-preferred partner (LPP) is a widely expressed protein in the heart, particularly in cardiac fibroblasts. It is found to be significantly elevated in patients with heart failure. LPP levels are elevated by suppressing nitric oxygen, however other fibroblast protein are not affected. This protein may be involved in cardiac homeostasis, as well as heart disease.

Biological assays

Many biological assays that utilize the LPP marker for proteins can be utilized. They can be used to identify programmed cell death and understand the activity of cells and their responses to the microenvironment. It is a great partner in the creation and validation of bioassays that are suitable for a variety applications because of its advanced cell-based assay capabilities as well as its highly experienced scientists. The company offers a wide array of cell-based tests that conform to regulatory and scientific standards.

Bioassays are complex instruments that require meticulous design and validation to achieve reproducible results. The selection of an appropriate reference standard is essential to the assay's success. Some companies choose a sample from their own R&D process to serve as an example of a reference standard. As the test progresses, a suitable reference sample will be chosen. The objective of the test will determine the reference standard.

Multiplexing is used to define LPP-based assays. Multiplexed tests can simultaneously test multiple body fluids, which is different from conventional chemical methods that are presumed to be. Bioassays utilizing the LPP marker can be used to identify the drug being targeted and to determine the risk of biohazards. These tests are essential for regulatory submissions of biopharmaceutical product.

Bioinformatics and molecular biology experts recommend that the LPP marker be used to conduct high-quality bioassays for hemotopoetic products. It can also be used to identify growth factors that affect cells attached to substrates. For biopotency tests, experts in molecular biology advise freezing or purifying PBMC populations.

Apart from being a helpful marker for animal disease diagnostics In addition, LPP can be used for the identification of five terrestrial Brucella species. HRM multiplex provides diagnostic advantages over SNP-based typing. It can also be used with Brucella whole-genome sequencing. It's an affordable and easy alternative to hydrolysis probe-based species-defining tests. The software of the company allows researchers to detect a wide range of bacterial species.

To investigate the function LPP plays in the direction of cognitive emotion regulation in children, biological assays made with LPP markers aren't often used. LPP is simple to administer and provides high resolution in time. It is also more simple to administer than functional neuroimaging. The current study is based on a previous study which suggested LPP is sensitive to emotional responses to passive image viewing.

IHC

Boster Bio has over 16,000 antibodies that are validated for use in IHC, WB, ELISA, FC, and ELISA. They provide mouse monoclonal antibody and rabbit polyclonal antibodies. All antibodies come from Boster's own labs and Boster also offers a complimentary secondary antibody with every purchase. Their antisera are derived from mouse or human lyophilized proteins.

IHC in Boster Bio is a wonderful service for those looking to apply their research findings to diagnostic testing. An online guide to immunohistochemistry is now available. It offers tips and tricks to help you resolve common issues, such as weak staining or high background. Boster Bio has also selected key IHC protocols and methods to help you gain the most value from your IHC research.

Immunohistochemistry (IHC) is a well-known technique used in laboratories to visualize the distribution of cellular components inside tissues and within cells. It is possible to identify antigens using a variety of different IHC methods, and you can decide which is the most suitable for your research. The choice of the correct method is dependent on the type of tissue sample as well as the sensitivity of the test. Sample preparation is essential in the procedure because it determines the quality of the tissue sample which impacts the amount of antigen displaying.

The primary-secondary-ABC system is an effective and highly specific method. This method utilizes avidins which are coupled with signal molecules, usually Horseradish Peroxide (HRP), to target proteins of interest. This method offers high specificity and low background, which makes it a perfect choice for researchers. However, there are issues that make it not suitable for specific applications. Therefore, it is best to consult an expert before purchasing an IHC kit.

Functional variant of celiac disease

Researchers have recently reported a functional form of celiac disease in a group of Italian patients and healthy controls. The LPP gene is found in large numbers in the small intestine. This suggests that it could play an important role in cell motility and controlling the regulation. However, further investigation is needed to confirm the role played by this gene in the disease. These findings suggest that it could be a causal gene for the disease.

The LPP marker is used successfully to identify individuals suffering from celiac disease and its related condition, gluten-sensitive enteropathy. These markers are derived from tissue Transglutaminase which is a protein that the immune system recognizes as an autoantigen. The condition may be caused by environmental factors. The disease is prevalent among people of all ages and manifests with a variety symptoms such as extra- and subclinical.

The coexpression levels of genes vary from higher to lower in non-celiac patients and controls at diagnosis. The coexpression levels of 18 genes are highest in the two groups of patients and lower in the non-celiac control group. These genes were co-expressed by three SNPs (rs1018326, the chromosome 2). These genes are in control of three SNPs in celiac disease.

Deamidated anti-gliadin peptides are accurate indicators of coeliac disease the early stages of life. Synthetic neoepitopes of the transglutaminase-deamidated gliadin peptide complex are also useful as biomarkers for celiac disease. Deamidated gliadin-deamidated peptide antibodies can be found in human umbilical cord tissue.