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Product Info Summary
| SKU: | PA2263 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-PRMT4/CARM1 Antibody Picoband®
SKU/Catalog Number
PA2263
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-PRMT4/CARM1 Antibody catalog # PA2263. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PRMT4/CARM1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2263)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human PRMT4, identical to the related mouse and rat sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2263 is reactive to CARM1 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
60 kDa
Calculated molecular weight
65854 MW
Background of CARM1
CARM1 (coactivator-associated arginine methyltransferase 1), also known as PRMT4, is an enzyme encoded by the CARM1 gene found in human beings, as well as many other mammals. This gene is mapped to 19p13.2. CARM1 is a regulator of cyclin E1 and DHFR mRNA expression. Its main function includes catalyzing the transfer of a methyl group from S-adenosyl-L-methionine to the side chain nitrogens of arginine residues within proteins to form methylated arginine derivatives and S-adenosyl-L-homocysteine. CARM1 is a secondary coactivator through its association with p160 family (SRC-1, GRIP1, AIB) of coactivators. It is responsible for moving cells toward the inner cell mass in developing blastocysts. This gene also plays an important role in androgen receptors and may play a role in prostate cancer progression.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA2263 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
Positive Control
WB: Rat Spleen Tissue, Human Placenta Tissue, Rat Kidney Tissue, NIH Whole Cell, HELA Whole Cell, HL60 Whole Cell, JURKAT Whole Cell
Validation Images & Assay Conditions
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Anti-PRMT4 antibody, PA2263, All Western blotting
All lanes: Anti-CARM1(PA2263) at 0.5ug/ml
Lane 1: Rat Spleen Tissue Lysate at 40ug
Lane 2: Human Placenta Tissue Lysate at 40ug
Lane 3: Rat Kidney Tissue Lysate at 40ug
Lane 4: NIH Whole Cell Lysate at 40ug
Lane 5: HELA Whole Cell Lysate at 40ug
Lane 6: HL60 Whole Cell Lysate at 40ug
Lane 7: JURKAT Whole Cell Lysate at 40ug
Predicted bind size: 60KD
Observed bind size: 60KD
Protein Target Info & Infographic
Gene/Protein Information For CARM1 (Source: Uniprot.org, NCBI)
Gene Name
CARM1
Full Name
Histone-arginine methyltransferase CARM1
Weight
65854 MW
Superfamily
class I-like SAM-binding methyltransferase superfamily
Alternative Names
Histone-arginine methyltransferase CARM1;2.1.1.-;2.1.1.125;Coactivator-associated arginine methyltransferase 1;Protein arginine N-methyltransferase 4;CARM1;PRMT4; CARM1 PRMT4 coactivator associated arginine methyltransferase 1 histone-arginine methyltransferase CARM1|protein arginine N-methyltransferase 4
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CARM1, check out the CARM1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CARM1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PRMT4/CARM1 Antibody Picoband® (PA2263)
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No publications found for PA2263
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Customer Q&As
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1 Customer Q&As for Anti-PRMT4/CARM1 Antibody Picoband®
Question
We are currently using anti-PRMT4/CARM1 antibody PA2263 for rat tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2019-11-08
Answer
The anti-PRMT4/CARM1 antibody (PA2263) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-08
