Product Info Summary
SKU: | A12184 |
---|---|
Size: | 100 µl |
Reactive Species: | Human |
Host: | Rabbit |
Application: | IHC |
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Product info
Product Name
Anti-CDKL3/Nkiamre Antibody
SKU/Catalog Number
A12184
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-CDKL3/Nkiamre Antibody (Catalog # A12184). Tested in IHC applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CDKL3/Nkiamre Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12184)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human CDKL3.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A12184 is reactive to CDKL3 in Human
Applications
A12184 is guaranteed for IHC Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
67514 MW
Background of NKIAMRE
The protein encoded by this gene is a member of cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of Saccharomyces cerevisiae cdc28, and Schizosaccharomyces pombe cdc2, and are known to be important regulators of cell cycle progression. This gene was identified as a gene absent in leukemic patients with chromosome 5q deletion. This loss may be an important determinant of dysmyelopoiesis. Alternative splicing results in multiple transcript variants encoding different isoforms.
Midmer M., Submitted (FEB-1999) to the EMBL/GenBank/DDBJ databases.
The MGC Project Team, Genome Res. 14:2121-2127(2004).
Greenman C., Nature 446:153-158(2007).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry: 1:50~1:100
Validation Images & Assay Conditions
Click image to see more details
Immunohistochemistry analysis of paraffin-embedded human skeletal muscle tissue, using CDKL3 antibody A12184.
Protein Target Info & Infographic
Gene/Protein Information For CDKL3 (Source: Uniprot.org, NCBI)
Gene Name
CDKL3
Full Name
Cyclin-dependent kinase-like 3
Weight
67514 MW
Superfamily
protein kinase superfamily
Alternative Names
cyclin-dependent kinase-like 3; EC 2.7.11; EC 2.7.11.22; NKIAMRESerine/threonine-protein kinase NKIAMRE; serine-threonine protein kinase NKIAMRE CDKL3 NKIAMRE cyclin dependent kinase like 3 cyclin-dependent kinase-like 3|serine-threonine protein kinase NKIAMRE
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CDKL3, check out the CDKL3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CDKL3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CDKL3/Nkiamre Antibody (A12184)
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No publications found for A12184
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-CDKL3/Nkiamre Antibody
Question
I have attached the WB image, lot number and protocol we used for testis using anti-CDKL3/Nkiamre antibody A12184. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-08-13
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-13
Question
I was wanting to use your anti-CDKL3/Nkiamre antibody for IHC for human testis on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human testis identification?
Verified Customer
Verified customer
Asked: 2019-06-14
Answer
It shows on the product datasheet, A12184 anti-CDKL3/Nkiamre antibody has been tested for IHC on human tissues. We have an innovator award program that if you test this antibody and show it works in human testis in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-06-14
Question
Is a blocking peptide available for product anti-CDKL3/Nkiamre antibody (A12184)?
P. Yang
Verified customer
Asked: 2018-01-24
Answer
We do provide the blocking peptide for product anti-CDKL3/Nkiamre antibody (A12184). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-01-24
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for testis using anti-CDKL3/Nkiamre antibody A12184. Let me know if you need anything else.
M. Banerjee
Verified customer
Asked: 2017-03-13
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-03-13
Question
We are currently using anti-CDKL3/Nkiamre antibody A12184 for human tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on zebrafish tissues as well?
C. Evans
Verified customer
Asked: 2016-12-26
Answer
The anti-CDKL3/Nkiamre antibody (A12184) has not been tested for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-12-26
Question
We want to test anti-CDKL3/Nkiamre antibody A12184 on human testis for research purposes, then I may be interested in using anti-CDKL3/Nkiamre antibody A12184 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
J. Bhatt
Verified customer
Asked: 2015-03-04
Answer
The products we sell, including anti-CDKL3/Nkiamre antibody A12184, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2015-03-04
Question
Would anti-CDKL3/Nkiamre antibody A12184 work on canine IHC with fetal heart?
A. Wu
Verified customer
Asked: 2015-02-27
Answer
Our lab technicians have not validated anti-CDKL3/Nkiamre antibody A12184 on canine. You can run a BLAST between canine and the immunogen sequence of anti-CDKL3/Nkiamre antibody A12184 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine fetal heart in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-02-27