Anti-Lin28 LIN28A Rabbit Monoclonal Antibody

Product Name

Anti-Lin28 LIN28A Rabbit Monoclonal Antibody

View all LIN-28A Antibodies

SKU/Catalog Number

M01966

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-Lin28 LIN28A Rabbit Monoclonal Antibody catalog # M01966. Tested in WB, IP, Flow Cytometry applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Lin28 LIN28A Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01966)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

AAAG-12

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human Lin28

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M01966 is reactive to LIN28A in Human

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Observed Molecular Weight

26 kDa

Calculated molecular weight

22743 MW

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M01966 is guaranteed for Flow Cytometry, IP, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IP 1:50
FC 1:50

Positive Control

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of Lin28 using anti-Lin28 antibody (M01966).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Caco-2 whole cell lysates,
Lane 2: human K562 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Lin28 antigen affinity purified monoclonal antibody (Catalog # M01966) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Lin28 at approximately 26 kDa. The expected band size for Lin28 is at 24 kDa.

Gene/Protein Information For LIN28A (Source: Uniprot.org, NCBI)

Alternative Names

CSDD1; CSDD1lin-28A; FLJ12457; lin-28 homolog (C. elegans); lin-28 homolog A (C. elegans); LIN28; LIN-28; LIN28A; LIN-28A; LIN28RNA-binding protein LIN-28; Tex17; ZCCHC1; ZCCHC1protein lin-28 homolog A; Zinc finger CCHC domain-containing protein 1; zinc finger, CCHC domain containing 1 LIN28A CSDD1, LIN-28, LIN28, ZCCHC1, lin-28A lin-28 homolog A protein lin-28 homolog A|RNA-binding protein LIN-28|zinc finger CCHC domain-containing protein 1|zinc finger, CCHC domain containing 1

For more info on LIN28A, check out the LIN28A Infographic

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In this infographic, you will see the following information for LIN28A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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