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Product Info Summary
| SKU: | A01966 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-Lin28 Antibody
SKU/Catalog Number
A01966
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Lin28 Antibody (Catalog # A01966). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human.
Storage & Handling
Lin28 antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Lin28 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01966)
Host
Rabbit
Contents
Lin28 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Lin28 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human Lin28. The immunogen is located within the last 50 amino acids of Lin28.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
SRPK1 antibody is human, mouse and rat reactive. Multiple isoforms of SRPK1 are known to exist; this antibody will detect all except isoforms b and e. SRPK1 antibody is predicted to not cross-react with SRPK2.
Reactive Species
A01966 is reactive to LIN28A in Human
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
22743 MW
Background of LIN-28A
Lin28 is a transcription factor that was first identified through its key role in the pathway of developmental timing in C. elegans. The role of Lin28 in development suggested that it might be useful in the creation of stem cells that might be beneficial in cell replacement therapies in the treatment of several degenerative diseases. Artificial stem cells, termed induced pluripotent stem (iPS) cells, can be created by expressing Lin28 in addition to the transcription factors POU5F1, Sox2, and NANOG in mouse fibroblasts. More recently, experiments have demonstrated that iPS cells could be generated using expression plasmids expressing Lin28, Sox2, POU5F1 and c-Myc, eliminating the need for virus introduction, thereby addressing a safety concern for potential use of iPS cells in regenerative medicine.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01966 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Lin28 antibody can be used for detection of Lin28 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of Lin28 in Raji cell lysate with Lin28 antibody at (A) 1 and (B) 2 μg/mL.
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Immunohistochemistry of KLF4 in human spleen tissue with KLF4 antibody at 5 μg/mL.
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Immunofluorescence of LIN28 in human spleen tissue with LIN28 antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For LIN28A (Source: Uniprot.org, NCBI)
Gene Name
LIN28A
Full Name
Protein lin-28 homolog A
Weight
22743 MW
Superfamily
lin-28 family
Alternative Names
CSDD1, LIN28, LIN-28, ZCCHC1, lin-28A, CSDD1, Protein lin-28 homolog A, Zinc finger CCHC domain-containing protein 1, Lin-28A LIN28A CSDD1, LIN-28, LIN28, ZCCHC1, lin-28A lin-28 homolog A protein lin-28 homolog A|RNA-binding protein LIN-28|zinc finger CCHC domain-containing protein 1|zinc finger, CCHC domain containing 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on LIN28A, check out the LIN28A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for LIN28A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Lin28 Antibody (A01966)
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No publications found for A01966
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