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Product Info Summary
| SKU: | M01966-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-LIN28 LIN28A Monoclonal Antibody
SKU/Catalog Number
M01966-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-LIN28 LIN28A Monoclonal Antibody catalog # M01966-1. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-LIN28 LIN28A Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01966-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AFFE-12
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human LIN28 Involved in the timing of developmental events and choice of stage specific cell fates. Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre-let-7.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01966-1 is reactive to LIN28A in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
33 kDa
Calculated molecular weight
22743 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01966-1 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:5000
IHC 1:50-1:200
ICC/IF 1:100-1:500
IP 1:50
FC 1:50
Positive Control
WB: NCCIT cell
Validation Images & Assay Conditions
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Western blot analysis of LIN28 expression in NCCIT cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For LIN28A (Source: Uniprot.org, NCBI)
Gene Name
LIN28A
Full Name
Protein lin-28 homolog A
Weight
22743 MW
Superfamily
lin-28 family
Alternative Names
Protein lin-28 homolog A;Lin-28A;Zinc finger CCHC domain-containing protein 1;LIN28A;CSDD1, LIN28, ZCCHC1; LIN28A CSDD1, LIN-28, LIN28, ZCCHC1, lin-28A lin-28 homolog A protein lin-28 homolog A|RNA-binding protein LIN-28|zinc finger CCHC domain-containing protein 1|zinc finger, CCHC domain containing 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on LIN28A, check out the LIN28A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for LIN28A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-LIN28 LIN28A Monoclonal Antibody (M01966-1)
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No publications found for M01966-1
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-LIN28 LIN28A Monoclonal Antibody
Question
What sample was tested in WB and IHC applications with M01966-1?
Verified customer
Asked: 2020-01-09
Answer
Western blot analysis of LIN28 expression in NCCIT cell lysate.
Boster Scientific Support
Answered: 2020-01-09
Question
Can you help my question with product M01966-1, anti-LIN28 Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-11-22
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01966-1 anti-LIN28 Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-11-22
Question
Does anti-LIN28 Monoclonal antibody M01966-1 work for WB with teratocarcinoma?
Verified Customer
Verified customer
Asked: 2019-07-19
Answer
According to the expression profile of teratocarcinoma, LIN28A is highly expressed in teratocarcinoma. So, it is likely that anti-LIN28 Monoclonal antibody M01966-1 will work for WB with teratocarcinoma.
Boster Scientific Support
Answered: 2019-07-19
Question
We are currently using anti-LIN28 Monoclonal antibody M01966-1 for human tissue, and we are well pleased with the IP results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2019-06-24
Answer
The anti-LIN28 Monoclonal antibody (M01966-1) has not been validated for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-06-24
