Click image to see more details
Product Info Summary
| SKU: | A01400 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-LIF Antibody Picoband®
SKU/Catalog Number
A01400
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-LIF Antibody Picoband® catalog # A01400. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-LIF Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01400)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived mouse LIF recombinant protein (Position: S24-F203).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01400 is reactive to Lif in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
18 kDa
Calculated molecular weight
22.008kDa
Background of LIF
LIF is a pleiotropic cytokine produced at the maternal-fetal interface which has been shown to play an essential role in implantation in mice. This gene is mapped to 22q11-q12.2, between the Philadelphia translocation BCR gene and the breakpoint of the translocation in cell line GM2324 at 22q12.2. LIF is produced in high amounts by the human endometrium and the trophoblast itself, and LIF receptors are present on cytotrophoblast cells. LIF could, thus, play a role in modulating HLA-G production and immune tolerance at the maternal-fetal interface.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01400 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
ELISA (Cap), 1-5μg/ml
Positive Control
WB: rat brain tissue, rat heart tissue, rat liver tissue, rat kidney tissue, human Hela cell, human placenta tissue, human Jurkat cell, mouse liver tissuemouse kidney tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of LIF using anti-LIF antibody (A01400).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: rat heart tissue lysates,
Lane 3: rat liver tissue lysates,
Lane 4: rat kidney tissue lysates,
Lane 5: human Hela cell lysates,
Lane 6: human placenta tissue lysates,
Lane 7: human Jurkat cell lysates,
Lane 8: mouse liver tissue lysates.
Lane 9: mouse kidney tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LIF antigen affinity purified polyclonal antibody (Catalog # A01400) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LIF at approximately 18KD. The expected band size for LIF is at 22KD.
Protein Target Info & Infographic
Gene/Protein Information For Lif (Source: Uniprot.org, NCBI)
Gene Name
Lif
Full Name
Leukemia inhibitory factor
Weight
22.008kDa
Superfamily
LIF/OSM family
Alternative Names
Leukemia inhibitory factor; LIF; Differentiation-stimulating factor; D factor; Lif LIF CDF, DIA, HILDA, MLPLI LIF interleukin 6 family cytokine leukemia inhibitory factor|D factor|cholinergic differentiation factor|differentiation inhibitory activity|differentiation-inducing factor|differentiation-stimulating factor|hepatocyte-stimulating factor III|human interleukin in DA cells|melanoma-derived LPL inhibitor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Lif, check out the Lif Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Lif: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-LIF Antibody Picoband® (A01400)
Hello CJ!
A01400 has been cited in 4 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Optimization of Culture Conditions to Support Undifferentiated Growth of Human Embryonic Stem Cells
miR-182 aids in receptive endometrium development in dairy goats by down-regulating PTN expression
MicroRNA-223-3p suppresses leukemia inhibitory factor expression and pinopodes formation during embryo implantation in mice
Hu Cp, Feng Jt, Tang Yl, Zhu Jq, Lin Mj, Yu Me. Mediators Inflamm. 2006;2006(5):84829. Lif Upregulates Expression Of Nk-1R In Nhbe Cells.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-LIF Antibody Picoband®?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-LIF Antibody Picoband®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
16 Customer Q&As for Anti-LIF Antibody Picoband®
Question
Can you help my question with product A01400, anti-LIF antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-03-02
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A01400 anti-LIF antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-03-02
Question
We are currently using anti-LIF antibody A01400 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on zebrafish tissues as well?
D. Williams
Verified customer
Asked: 2020-02-10
Answer
The anti-LIF antibody (A01400) has not been tested for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-02-10
Question
I see that the anti-LIF antibody A01400 works with ELISA, what is the protocol used to produce the result images on the product page?
B. Edwards
Verified customer
Asked: 2019-09-11
Answer
You can find protocols for ELISA on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-09-11
Question
We have tried in the past anti-LIF antibody for WB on colon in a previous project. I am using mouse, and I plan to use the antibody for ELISA next. Our lab want to know about examining colon as well as left coronary artery in our next experiment. Could give a recommendation on which antibody would work the best for ELISA?
Verified Customer
Verified customer
Asked: 2019-08-06
Answer
I have checked the website and datasheets of our anti-LIF antibody and it appears that A01400 has been tested on mouse in both WB and ELISA. Thus A01400 should work for your application. Our Boster satisfaction guarantee will cover this product for ELISA in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for ELISA detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-08-06
Question
My boss were well pleased with the WB result of your anti-LIF antibody. However we have seen positive staining in left coronary artery secreted. using this antibody. Is that expected? Could you tell me where is LIF supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-07-11
Answer
From what I have seen in literature, left coronary artery does express LIF. Generally LIF expresses in secreted. Regarding which tissues have LIF expression, here are a few articles citing expression in various tissues:
Colon, Pubmed ID: 15489334
Embryonic stem cell, Pubmed ID: 15146197
Boster Scientific Support
Answered: 2019-07-11
Question
I would like to test anti-LIF antibody A01400 on rat embryonic stem cell for research purposes, then I may be interested in using anti-LIF antibody A01400 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-06-27
Answer
The products we sell, including anti-LIF antibody A01400, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-06-27
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for embryonic stem cell using anti-LIF antibody A01400. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-06-19
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-19
Question
We want using your anti-LIF antibody for negative regulation of erk1 and erk2 cascade studies. Has this antibody been tested with western blotting on kidney tissue? We would like to see some validation images before ordering.
E. Patel
Verified customer
Asked: 2018-11-29
Answer
We appreciate your inquiry. This A01400 anti-LIF antibody is validated on rat heart tissue, brain tissue, liver tissue, kidney tissue, human placenta tissue, jurkat cell lysates, mouse liver tissue. It is guaranteed to work for ELISA, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2018-11-29
Question
Is this A01400 anti-LIF antibody reactive to the isotypes of LIF?
Verified Customer
Verified customer
Asked: 2018-07-24
Answer
The immunogen of A01400 anti-LIF antibody is E. coli-derived mouse LIF recombinant protein (Position: S24-F203). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-07-24
Question
See below the WB image, lot number and protocol we used for embryonic stem cell using anti-LIF antibody A01400. Please let me know if you require anything else.
C. Banerjee
Verified customer
Asked: 2017-08-21
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-08-21
Question
Will A01400 anti-LIF antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
N. Jackson
Verified customer
Asked: 2016-03-31
Answer
As indicated on the product datasheet, A01400 anti-LIF antibody as been tested on ELISA. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2016-03-31
Question
Is a blocking peptide available for product anti-LIF antibody (A01400)?
Z. Johnson
Verified customer
Asked: 2016-02-15
Answer
We do provide the blocking peptide for product anti-LIF antibody (A01400). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2016-02-15
Question
Would anti-LIF antibody A01400 work for ELISA with embryonic stem cell?
N. Carter
Verified customer
Asked: 2015-02-11
Answer
According to the expression profile of embryonic stem cell, LIF is highly expressed in embryonic stem cell. So, it is likely that anti-LIF antibody A01400 will work for ELISA with embryonic stem cell.
Boster Scientific Support
Answered: 2015-02-11
Question
We have seen staining in rat embryonic stem cell. Are there any suggestions? Is anti-LIF antibody supposed to stain embryonic stem cell positively?
D. Parker
Verified customer
Asked: 2015-02-02
Answer
Based on literature embryonic stem cell does express LIF. Based on Uniprot.org, LIF is expressed in left coronary artery, embryonic stem cell, colon, among other tissues. Regarding which tissues have LIF expression, here are a few articles citing expression in various tissues:
Colon, Pubmed ID: 15489334
Embryonic stem cell, Pubmed ID: 15146197
Boster Scientific Support
Answered: 2015-02-02
Question
I was wanting to use your anti-LIF antibody for ELISA for rat embryonic stem cell on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat embryonic stem cell identification?
K. Carter
Verified customer
Asked: 2013-11-01
Answer
As indicated on the product datasheet, A01400 anti-LIF antibody has been tested for ELISA, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat embryonic stem cell in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-11-01
Question
Is there a BSA free version of anti-LIF antibody A01400 available?
D. Miller
Verified customer
Asked: 2013-01-11
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-LIF antibody A01400 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2013-01-11
