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Product Info Summary
| SKU: | PA1075 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-STAT1 Antibody Picoband®
SKU/Catalog Number
PA1075
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-STAT1 Antibody catalog # PA1075. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-STAT1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1075)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human STAT1, different from the related mouse sequence by one amino acid.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1075 is reactive to STAT1 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
87 kDa
Calculated molecular weight
87335 MW
Background of STAT1
Signal transducer and activator of transcription 1 (STAT1) is a transcription factor which in humans is encoded by the STAT1 gene. The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein can be activated by various ligands including interferon-alpha, interferon-gamma, EGF, PDGF and IL6. This protein mediates the expression of a variety of genes, which is thought to be important for cell viability in response to different cell stimuli and pathogens. Two alternatively spliced transcript variants encoding distinct isoforms have been described.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1075 is guaranteed for Flow Cytometry, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry(Fixed), 1-3 μg/1x106 cells, Human
Positive Control
WB: human A549 whole cell, human Hela whole cell, human 293T whole cell, human K562 whole cell, rat PC-12 whole cell, rat NRK whole cell, mouse NIH/3T3 whole cell
ICC/IF: Hela cell
b>FCM: A549 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of STAT1 using anti-STAT1 antibody (PA1075).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A549 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human 293T whole cell lysates,
Lane 4: human K562 whole cell lysates,
Lane 5: rat PC-12 whole cell lysates,
Lane 6: rat NRK whole cell lysates,
Lane 7: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STAT1 antigen affinity purified polyclonal antibody (Catalog # PA1075) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for STAT1 at approximately 87 kDa. The expected band size for STAT1 is at 87 kDa.
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Figure 2. IF analysis of STAT1 using anti-STAT1 antibody (PA1075).
STAT1 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-STAT1 Antibody (PA1075) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Click image to see more details
Figure 3. Flow Cytometry analysis of A549 cells using anti-STAT1 antibody (PA1075).
Overlay histogram showing A549 cells stained with PA1075 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-STAT1 Antibody (PA1075, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For STAT1 (Source: Uniprot.org, NCBI)
Gene Name
STAT1
Full Name
Signal transducer and activator of transcription 1-alpha/beta
Weight
87335 MW
Superfamily
transcription factor STAT family
Alternative Names
Signal transducer and activator of transcription 1-alpha/beta;Transcription factor ISGF-3 components p91/p84;STAT1; STAT1 CANDF7, IMD31A, IMD31B, IMD31C, ISGF-3, STAT91 signal transducer and activator of transcription 1 signal transducer and activator of transcription 1-alpha/beta|signal transducer and activator of transcription 1, 91kD|signal transducer and activator of transcription 1, 91kDa|transcription factor ISGF-3 components p91/p84
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on STAT1, check out the STAT1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for STAT1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-STAT1 Antibody Picoband® (PA1075)
Hello CJ!
PA1075 has been cited in 4 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Synergistic inhibition of colon cancer growth by the combination of methylglyoxal and silencing of glyoxalase I mediated by the STAT1 pathway
Hepatitis C virus non-structural 5A abrogates signal transducer and activator of transcription-1 nuclear translocation induced by IFN-alpha through dephosphorylation.
Hydrogen sulfide attenuates myocardial fibrosis in diabetic rats through the JAK/STAT signaling pathway
Song Cg, Yang X, Min Lq, Liu Cx, Zhao Cs. Neuro Endocrinol Lett. 2014;35(1):68-72. The Effect Of Procyanidin On Expression Of Stat1 In Type 2 Diabetes Mellitus Sd Rats With Focal Cerebral Ischemia.
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Customer Q&As
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16 Customer Q&As for Anti-STAT1 Antibody Picoband®
Question
I was wanting to use to test anti-STAT1 antibody PA1075 on mouse cervix carcinoma for research purposes, then I may be interested in using anti-STAT1 antibody PA1075 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
M. Parker
Verified customer
Asked: 2020-04-28
Answer
The products we sell, including anti-STAT1 antibody PA1075, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-04-28
Question
We want using your anti-STAT1 antibody for response to peptide hormone studies. Has this antibody been tested with western blotting on thymus tissue? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2020-02-10
Answer
Thank you for your inquiry. This PA1075 anti-STAT1 antibody is tested on hela cell lysate, mouse ovary tissue, heart tissue, tissue lysate, liver tissue, brain tissue, kidney tissue, spleen tissue, thymus tissue, lung tissue, intestine tissue, rat thymus tissue, testis tissue. It is guaranteed to work for WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-02-10
Question
Is this PA1075 anti-STAT1 antibody reactive to the isotypes of STAT1?
Verified Customer
Verified customer
Asked: 2020-01-31
Answer
The immunogen of PA1075 anti-STAT1 antibody is A synthetic peptide corresponding to a sequence in the middle region of human STAT1(364-378aa FDKDVNERNTVKGFR), different from the related mouse sequence by one amino acid. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-01-31
Question
Would PA1075 anti-STAT1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-12-19
Answer
You can see on the product datasheet, PA1075 anti-STAT1 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-12-19
Question
I have a question about product PA1075, anti-STAT1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-12-09
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1075 anti-STAT1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-12-09
Question
Is there a BSA free version of anti-STAT1 antibody PA1075 available?
Verified Customer
Verified customer
Asked: 2019-09-25
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-STAT1 antibody PA1075 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-09-25
Question
Please see the WB image, lot number and protocol we used for cervix carcinoma using anti-STAT1 antibody PA1075. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-09-19
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-09-19
Question
Our team were happy with the WB result of your anti-STAT1 antibody. However we have observed positive staining in cervix carcinoma cytoplasm using this antibody. Is that expected? Could you tell me where is STAT1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-09-06
Answer
According to literature, cervix carcinoma does express STAT1. Generally STAT1 expresses in cytoplasm. Regarding which tissues have STAT1 expression, here are a few articles citing expression in various tissues:
B-cell, Pubmed ID: 9724754
Brain, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Placenta, and Testis, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2019-09-06
Question
I was wanting to use your anti-STAT1 antibody for WB for mouse cervix carcinoma on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse cervix carcinoma identification?
Verified Customer
Verified customer
Asked: 2019-08-30
Answer
As indicated on the product datasheet, PA1075 anti-STAT1 antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse cervix carcinoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-30
Question
Would anti-STAT1 antibody PA1075 work for WB with cervix carcinoma?
Verified Customer
Verified customer
Asked: 2019-07-01
Answer
According to the expression profile of cervix carcinoma, STAT1 is highly expressed in cervix carcinoma. So, it is likely that anti-STAT1 antibody PA1075 will work for WB with cervix carcinoma.
Boster Scientific Support
Answered: 2019-07-01
Question
I see that the anti-STAT1 antibody PA1075 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-06-21
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-06-21
Question
Is a blocking peptide available for product anti-STAT1 antibody (PA1075)?
Verified Customer
Verified customer
Asked: 2019-06-12
Answer
We do provide the blocking peptide for product anti-STAT1 antibody (PA1075). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-06-12
Question
We are currently using anti-STAT1 antibody PA1075 for rat tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2018-10-29
Answer
The anti-STAT1 antibody (PA1075) has not been validated for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-10-29
Question
We have observed staining in human epithelium of bronchus. Do you have any suggestions? Is anti-STAT1 antibody supposed to stain epithelium of bronchus positively?
C. Anderson
Verified customer
Asked: 2017-08-29
Answer
From literature epithelium of bronchus does express STAT1. From Uniprot.org, STAT1 is expressed in epithelium of bronchus, placenta testis, brain, b-cell, cervix carcinoma, leukemic t-cell, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have STAT1 expression, here are a few articles citing expression in various tissues:
B-cell, Pubmed ID: 9724754
Brain, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Placenta, and Testis, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2017-08-29
Question
Would anti-STAT1 antibody PA1075 work on pig WB with leukemic t-cell?
Verified Customer
Verified customer
Asked: 2017-07-03
Answer
Our lab technicians have not tested anti-STAT1 antibody PA1075 on pig. You can run a BLAST between pig and the immunogen sequence of anti-STAT1 antibody PA1075 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig leukemic t-cell in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-07-03
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma using anti-STAT1 antibody PA1075. Let me know if you need anything else.
R. Gonzalez
Verified customer
Asked: 2016-04-01
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-04-01
