Click image to see more details
Product Info Summary
| SKU: | RP1106 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IHC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-LFA3/CD58 Antibody Picoband®
View all CD58/LFA-3 Antibodies
SKU/Catalog Number
RP1106
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-LFA3/CD58 Antibody catalog # RP1106. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-LFA3/CD58 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1106)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human LFA3 recombinant protein (Position: F29-R215).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
RP1106 is reactive to CD58 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
26 kDa
Calculated molecular weight
28147 MW
Background of CD58/LFA-3
CD58, or lymphocyte function-associated antigen 3 (LFA-3), is a cell adhesion molecule expressed on Antigen Presenting Cells (APC), particularly macrophages. It binds to CD2 (LFA-2) on T cells and is important in strengthening the adhesion between the T cells and Professional Antigen Presenting Cells. This adhesion occurs as part of the transitory initial encounters between T cells and Antigen Presenting Cells before T cell activation, when T cells are roaming the lymph nodes looking at the surface of APCs for peptide:MHC complexes the T-cell receptors are reactive to. The LFA3 gene is mapped to chromosome 1p13, which is the same location as CD2.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
RP1106 is guaranteed for Flow Cytometry, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, By Heat
Flow Cytometry, 1-3μg/1x106cells
Positive Control
WB: HELA whole cell (human tonsil tissue
FCM: Hela cell
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of LFA3 expression in HELA whole cell lysates (lane 1) and K562 whole cell lysates (lane 2). LFA3 at 35KD was detected using rabbit anti-LFA3 Antigen Affinity purified polyclonal antibody (Catalog # RP1106) at 0.5 μg/mL. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).
Click image to see more details
LFA3 was detected in paraffin-embedded sections of human tonsil tissues using rabbit anti-LFA3 Antigen Affinity purified polyclonal antibody (Catalog # RP1106) at 1 μg/mL. The immunohistochemical section was developed using SABC method (Catalog # SA1022).
Click image to see more details
Figure 3. Flow Cytometry analysis of Hela cells using anti-LFA3 antibody (RP1106).
Overlay histogram showing Hela cells stained with RP1106 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-LFA3 Antibody (RP1106,1μg/1x106 cells) for 30 min at 20°C. DyLight488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For CD58 (Source: Uniprot.org, NCBI)
Gene Name
CD58
Full Name
Lymphocyte function-associated antigen 3
Weight
28147 MW
Alternative Names
Lymphocyte function-associated antigen 3;Ag3;Surface glycoprotein LFA-3;CD58;CD58;LFA3; CD58 LFA-3, LFA3, ag3 CD58 molecule lymphocyte function-associated 3|CD58 , (lymphocyte function-associated 3)|surface glycoprotein LFA-3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD58, check out the CD58 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD58: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-LFA3/CD58 Antibody Picoband® (RP1106)
Hello CJ!
No publications found for RP1106
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-LFA3/CD58 Antibody Picoband®?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-LFA3/CD58 Antibody Picoband®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
3 Customer Q&As for Anti-LFA3/CD58 Antibody Picoband®
Question
Would RP1106 anti-LFA3/CD58 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-03-16
Answer
You can see on the product datasheet, RP1106 anti-LFA3/CD58 antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-03-16
Question
I have attached the WB image, lot number and protocol we used for erythrocyte using anti-LFA3/CD58 antibody RP1106. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-10-30
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-30
Question
We are currently using anti-LFA3/CD58 antibody RP1106 for human tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2018-12-26
Answer
The anti-LFA3/CD58 antibody (RP1106) has not been tested for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-12-26
