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Product Info Summary
| SKU: | A05092 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IF, WB |
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Product info
Product Name
Anti-Leucyl-cystinyl aminopeptidase LNPEP Antibody
View all Leucyl-cystinyl Aminopeptidase/LNPEP Antibodies
SKU/Catalog Number
A05092
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Leucyl-cystinyl aminopeptidase LNPEP Antibody (Catalog # A05092). Tested in ELISA, WB, IF applications. This antibody reacts with Human.
Storage & Handling
LNPEP antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Leucyl-cystinyl aminopeptidase LNPEP Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05092)
Host
Rabbit
Contents
LNPEP Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
LNPEP antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human LNPEP. The immunogen is located within amino acids 900 - 950 of LNPEP.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
At least three isoforms are known to exist; this antibody will detect all three isoforms. Despite its predicted molecular weight, LNPEP often migrates at 165kD in SDS-PAGE.
Reactive Species
A05092 is reactive to LNPEP in Human
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
117349 MW
Background of Leucyl-cystinyl Aminopeptidase/LNPEP
LNPEP, also known as insulin-responsive aminopeptidase (IRAP), was initially identified as an abundant protein that is associated with specialized endosomes termed GLUT4 storage vesicles (GSVs) in adipocytes. The luminal domain of LNPEP contains an aminopeptidase activity that is involved in peptide hormone processing and antigenic peptide processing. LNPEP is also a key regulator of GLUT4 trafficking by controlling the sorting of GLUT4 from endosomes to the GSVs.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05092 is guaranteed for ELISA, IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
LNPEP antibody can be used for detection of LNPEP by Western blot at 1 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of LNPEP in human lung tissue lysate with LNPEP antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
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Immunofluorescence of LNPEP in human lung tissue with LNPEP antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For LNPEP (Source: Uniprot.org, NCBI)
Gene Name
LNPEP
Full Name
Leucyl-cystinyl aminopeptidase
Weight
117349 MW
Superfamily
peptidase M1 family
Alternative Names
CAP, IRAP, PLAP, P-LAP, OTASE, Leucyl-cystinyl aminopeptidase, Insulin-regulated membrane aminopeptidase, Cystinyl aminopeptidase LNPEP CAP, IRAP, P-LAP, PLAP leucyl and cystinyl aminopeptidase leucyl-cystinyl aminopeptidase|AT (4) receptor|angiotensin IV receptor|cystinyl aminopeptidase|insulin-regulated aminopeptidase|insulin-regulated membrane aminopeptidase|insulin-responsive aminopeptidase|otase|oxytocinase|placental leucine aminopeptidase|vasopressinase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on LNPEP, check out the LNPEP Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for LNPEP: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Leucyl-cystinyl aminopeptidase LNPEP Antibody (A05092)
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