Click image to see more details
Product Info Summary
| SKU: | A00002-4 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-TNF alpha Antibody Picoband®
SKU/Catalog Number
A00002-4
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-TNF alpha Antibody Picoband® catalog # A00002-4. Tested in WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TNF alpha Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00002-4)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of mouse TNF alpha, which shares 92.3% and 86.7% amino acid (aa) sequence identity with human and rat TNF alpha, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00002-4 is reactive to Tnf in Mouse
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
25 kDa
Calculated molecular weight
25896 MW
Background of TNF-alpha
TNFα (Tumor Necrosis Factor alpha) gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. And this cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. Moreover, this cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00002-4 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Mouse
Positive Control
WB: mouse spleen tissue, mouse thymus tissue, mouse liver tissue, mouse lung tissue, mouse kidney tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of TNF alpha using anti-TNF alpha antibody (A00002-4).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: mouse spleen tissue lysates,
Lane 2: mouse thymus tissue lysates,
Lane 3: mouse liver tissue lysates,
Lane 4: mouse lung tissue lysates,
Lane 5: mouse kidney tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNF alpha antigen affinity purified polyclonal antibody (Catalog # A00002-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TNF alpha at approximately 25KD. The expected band size for TNF alpha is at 25KD.
Protein Target Info & Infographic
Gene/Protein Information For Tnf (Source: Uniprot.org, NCBI)
Gene Name
Tnf
Full Name
Tumor necrosis factor
Weight
25896 MW
Superfamily
Tumor necrosis factor family
Alternative Names
Tumor necrosis factor;Cachectin;TNF-alpha;Tumor necrosis factor ligand superfamily member 2;TNF-a;Tumor necrosis factor, membrane form;N-terminal fragment;NTF;Intracellular domain 1;ICD1;Intracellular domain 2;ICD2;C-domain 1;C-domain 2;Tumor necrosis factor, soluble form;Tnf;Tnfa, Tnfsf2; TNF DIF-alpha, TNFA, TNFSF2, TNLG1F, TNF tumor necrosis factor tumor necrosis factor|APC1 protein|TNF, macrophage-derived|TNF, monocyte-derived|TNF-a|tumor necrosis factor ligand 1F|tumor necrosis factor ligand superfamily member 2|tumor necrosis factor-alpha
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Tnf, check out the Tnf Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Tnf: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TNF alpha Antibody Picoband® (A00002-4)
Hello CJ!
A00002-4 has been cited in 17 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Liao L,Huang L,Wei X,Yin L,Wei X,Li T.Bioinformatic and biochemical studies of formononetin against liver injure.Life Sci.2021 Feb 16:119229.doi:10.1016/j.lfs.2021.119229.Epub ahead of print.PMID:33607154.
Species: Mouse
Liao L,Huang L,Wei X,Yin L,Wei X,Li T.Bioinformatic and biochemical studies of formononetin against liver injure.Life Sci.2021 Feb 16:119229.doi:10.1016/j.lfs.2021.119229.Epub ahead of print.PMID:33607154.
Species: Mouse
Ye D,Hu Y,Zhu N,Gu W,Long G,Tao E,Fang M,Jiang M. Exploratory Investigation of Intestinal Structure and Function after Stroke in Mice. Mediators Inflamm.2021 Feb 15;2021:1315797.doi: 10.1155/2021/1315797.PMID:33642941;PMCID:PMC7902147.
Species: Mouse
A00002-4 usage in article: APP:WB, SAMPLE:INTESTINE TISSUE, DILUTION:1:400
Li Y,Zheng D,Shen D,Zhang X,Zhao X,Liao H.Protective Effects of Two Safflower Derived Compounds, Kaempferol and Hydroxysafflor Yellow A, on Hyperglycaemic Stress-Induced Podocyte Apoptosis via Modulating of Macrophage M1/M2 Polarization.J Immunol Res.2020
Species: Mouse
A00002-4 usage in article: APP: WB, SAMPLE:MACROPHAGES, DILUTION:1:500
Resveratrol attenuates hyperoxia%u2010induced oxidative stress, inflammation and fibrosis and suppresses Wnt/%u03B2%u2010catenin signalling in lungs of neonatal rats
Efficacy of acetylshikonin in preventing obesity and hepatic steatosis in db/db mice
Protective effects of baicalin on carbon tetrachloride induced liver injury by activating PPAR%u03B3 and inhibiting TGF%u03B21
Immunomodulatory properties of quercetin-3-O-%u03B1-L-rhamnopyranoside from Rapanea melanophloeos against influenza a virus
Neuroprotective effects of %u03B1-lipoic acid on long-term experimental autoimmune encephalomyelitis.
Polymeric micelles for potentiated antiulcer and anticancer activities of naringin
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-TNF alpha Antibody Picoband®
Question
I am interested in using your anti-TNF alpha antibody for positive regulation of interleukin-6 production studies. Has this antibody been tested with western blotting on liver tissue? We would like to see some validation images before ordering.
R. Mitchell
Verified customer
Asked: 2020-02-13
Answer
I appreciate your inquiry. This A00002-4 anti-TNF alpha antibody is validated on mouse thymus tissue, spleen tissue, liver tissue, lung tissue, kidney tissue. It is guaranteed to work for WB in mouse. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-02-13
Question
We are currently using anti-TNF alpha antibody A00002-4 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says mouse. Is it possible that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2019-08-28
Answer
The anti-TNF alpha antibody (A00002-4) has not been tested for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-08-28
Question
We have been able to see staining in mouse leukocyte. Do you have any suggestions? Is anti-TNF alpha antibody supposed to stain leukocyte positively?
Verified Customer
Verified customer
Asked: 2018-01-18
Answer
According to literature leukocyte does express TNF. According to Uniprot.org, TNF is expressed in leukocyte, blood, prostatic carcinoma, among other tissues. Regarding which tissues have TNF expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 15489334
Prostatic carcinoma, Pubmed ID: 8597870, 10205166
Boster Scientific Support
Answered: 2018-01-18
Question
Does A00002-4 cross react with human samples?
Verified Customer
Verified customer
Asked: 2017-08-03
Answer
A00002-4 does not cross-react with human samples.
Boster Scientific Support
Answered: 2017-08-03
Question
My team were happy with the WB result of your anti-TNF alpha antibody. However we have observed positive staining in blood cell membrane using this antibody. Is that expected? Could you tell me where is TNF supposed to be expressed?
J. Li
Verified customer
Asked: 2016-08-12
Answer
Based on literature, blood does express TNF. Generally TNF expresses in cell membrane. Regarding which tissues have TNF expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 15489334
Prostatic carcinoma, Pubmed ID: 8597870, 10205166
Boster Scientific Support
Answered: 2016-08-12
