Anti-LEF1 Mouse Monoclonal Antibody [Clone ID: OTI10A7]

Product Name

Anti-LEF1 Mouse Monoclonal Antibody [Clone ID: OTI10A7]

View all Lef1 Antibodies

SKU/Catalog Number

M00605-2

Size

100 µl

Description

Boster Bio LEF1 mouse monoclonal antibody,clone OTI10A7. Catalog# M00605-2. Tested in IF, IHC, WB. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C as received.

Cite This Product

Anti-LEF1 Mouse Monoclonal Antibody [Clone ID: OTI10A7] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00605-2)

Host

Mouse

Contents

PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Clonality

Monoclonal

Clone Number

OTI10A7

Isotype

IgG2b

Immunogen

Full length human recombinant protein of human LEF1 (NP_057353) produced in HEK293T cell.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00605-2 is reactive to Lef1 in Human, Mouse, Rat

Applications

M00605-2 is guaranteed for IF, IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Validation Images & Assay Conditions

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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LEF1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LEF1 (1:500).

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Western blot analysis of extracts (35ug) from Colo205 cells by using anti-LEF1 monoclonal antibody (1:500).

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Western blot analysis of extracts (35ug) from 2 different tissues by using anti-LEF1 monoclonal antibody (1:500).

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Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-LEF1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min

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Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-LEF1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min

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Immunofluorescent staining of Colo205 cells using anti-LEF1 antibody (M00605-2/green

Gene/Protein Information For Lef1 (Source: Uniprot.org, NCBI)

Alternative Names

DKFZp586H0919; FLJ46390; LEF1; LEF-1; lymphoid enhancer-binding factor 1; T cell-specific transcription factor 1-alpha; TCF10; TCF1ALPHA; TCF1-alpha; TCF7L3 Lef1|3000002B05, AI451430, Lef-, Lef-1|lymphoid enhancer binding factor 1|lymphoid enhancer-binding factor 1

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In this infographic, you will see the following information for Lef1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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