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Product Info Summary
| SKU: | RP1072 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Transcription factor Sp1 SP1 Antibody Picoband®
SKU/Catalog Number
RP1072
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Transcription factor Sp1 SP1 Antibody catalog # RP1072. Tested in WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Transcription factor Sp1 SP1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1072)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2mg Na2HPO4, 0.05 mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human SP1, different from the related mouse and rat sequences by two amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
RP1072 is reactive to SP1 in Human, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
90 kDa
Calculated molecular weight
80693 MW
Background of SP1
Transcription factor Sp1, also known as specificity protein 1* is a protein that in humans is encoded by the SP1 gene. The protein encoded by this gene is a zinc finger transcription factor that binds to GC-rich motifs of many promoters. The encoded protein is involved in many cellular processes, including cell differentiation, cell growth, apoptosis, immune responses, response to DNA damage, and chromatin remodeling. Post-translational modifications such as phosphorylation, acetylation, glycosylation, and proteolytic processing significantly affect the activity of this protein, which can be an activator or a repressor. Three transcript variants encoding different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
RP1072 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Rat
Positive Control
WB: human Hela whole cell, human CACO-2 whole cell, human Jurkat whole cell, human COLO320 whole, rat testis tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of SP1 using anti-SP1 antibody (RP1072).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human CACO-2 whole cell lysates,
Lane 3: human Jurkat whole cell lysates,
Lane 4: human COLO320 whole lysates,
Lane 5: rat testis tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SP1 antigen affinity purified polyclonal antibody (Catalog # RP1072) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SP1 at approximately 90 kDa. The expected band size for SP1 is at 90 kDa.
Protein Target Info & Infographic
Gene/Protein Information For SP1 (Source: Uniprot.org, NCBI)
Gene Name
SP1
Full Name
Transcription factor Sp1
Weight
80693 MW
Superfamily
Sp1 C2H2-type zinc-finger protein family
Alternative Names
Transcription factor Sp1;SP1;TSFP1; SP1 Sp1 transcription factor transcription factor Sp1|specificity protein 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SP1, check out the SP1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Transcription factor Sp1 SP1 Antibody Picoband® (RP1072)
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Customer Q&As
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4 Customer Q&As for Anti-Transcription factor Sp1 SP1 Antibody Picoband®
Question
Our lab were happy with the WB result of your anti-SP1 antibody. However we have observed positive staining in brain testis nucleus. cytoplasm. note=nuclear location is using this antibody. Is that expected? Could you tell me where is SP1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2018-06-27
Answer
From literature, brain testis does express SP1. Generally SP1 expresses in nucleus. cytoplasm. note=nuclear location is. Regarding which tissues have SP1 expression, here are a few articles citing expression in various tissues:
Brain, and Testis, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 10973950, 16964243, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Colon, Pubmed ID: 10976766
Boster Scientific Support
Answered: 2018-06-27
Question
you antibody using your anti-SP1 antibody for regulation of cholesterol biosynthetic process studies. Has this antibody been tested with western blotting on hela whole cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2018-06-06
Answer
Thanks for your inquiry. This RP1072 anti-SP1 antibody is tested on hela whole cell lysate. It is guaranteed to work for WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2018-06-06
Question
We are currently using anti-SP1 antibody RP1072 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2017-07-19
Answer
The anti-SP1 antibody (RP1072) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-07-19
Question
We have seen staining in human colon. Do you have any suggestions? Is anti-SP1 antibody supposed to stain colon positively?
D. Evans
Verified customer
Asked: 2015-02-09
Answer
From literature colon does express SP1. From Uniprot.org, SP1 is expressed in nipple, brain testis, cervix carcinoma, colon, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have SP1 expression, here are a few articles citing expression in various tissues:
Brain, and Testis, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 10973950, 16964243, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Colon, Pubmed ID: 10976766
Boster Scientific Support
Answered: 2015-02-09
