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Product Info Summary
| SKU: | PB9786 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-LAR/PTPRF Antibody Picoband®
SKU/Catalog Number
PB9786
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-LAR/PTPRF Antibody Picoband® catalog # PB9786. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-LAR/PTPRF Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9786)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human LAR, different from the related mouse and rat sequences by four amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB9786 is reactive to PTPRF in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
240 kDa
Calculated molecular weight
212879 MW
Background of LAR/PTPRF
Receptor-type tyrosine-protein phosphatase F is an enzyme that in humans is encoded by the PTPRF gene. The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP possesses an extracellular region, a single transmembrane region, and two tandem intracytoplasmic catalytic domains, and thus represents a receptor-type PTP. The extracellular region contains three Ig-like domains, and nine non-Ig like domains similar to that of neural-cell adhesion molecule. This PTP was shown to function in the regulation of epithelial cell-cell contacts at adherents junctions, as well as in the control of beta-catenin signaling. An increased expression level of this protein was found in the insulin-responsive tissue of obese, insulin-resistant individuals, and may contribute to the pathogenesis of insulin resistance. Two alternatively spliced transcript variants of this gene, which encode distinct proteins, have been reported.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9786 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: HELA Whole Cell, A431 Whole Cell, A549 Whole Cell
IHC: human lung cancer tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of LAR using anti-LAR antibody (PB9786).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 40 ug of sample under reducing conditions.
Lane 1: HELA Whole Cell Lysate,
Lane 2: A431 Whole Cell Lysate,
Lane 3: A549 Whole Cell Lysate.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LAR antigen affinity purified polyclonal antibody (Catalog # PB9786) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LAR at approximately 240 kDa. The expected band size for LAR is at 240 kDa.
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Figure 2. IHC analysis of LAR using anti-LAR antibody (PB9786).
LAR was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-LAR Antibody (PB9786) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For PTPRF (Source: Uniprot.org, NCBI)
Gene Name
PTPRF
Full Name
Receptor-type tyrosine-protein phosphatase F
Weight
212879 MW
Superfamily
protein-tyrosine phosphatase family
Alternative Names
Receptor-type tyrosine-protein phosphatase F;3.1.3.48;Leukocyte common antigen related;LAR;PTPRF;LAR; PTPRF BNAH2, LAR protein tyrosine phosphatase receptor type F receptor-type tyrosine-protein phosphatase F|LCA-homolog|leukocyte -related (LAR) PTP receptor|leukocyte -related tyrosine phosphatase|leukocyte common related|protein tyrosine phosphatase, receptor type, F polypeptide|receptor-linked protein-tyrosine phosphatase LAR
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PTPRF, check out the PTPRF Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PTPRF: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-LAR/PTPRF Antibody Picoband® (PB9786)
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Customer Q&As
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5 Customer Q&As for Anti-LAR/PTPRF Antibody Picoband®
Question
Does anti-LAR/PTPRF antibody PB9786 work for WB with epithelium of mammary gland?
Verified Customer
Verified customer
Asked: 2020-05-06
Answer
According to the expression profile of epithelium of mammary gland, PTPRF is highly expressed in epithelium of mammary gland. So, it is likely that anti-LAR/PTPRF antibody PB9786 will work for WB with epithelium of mammary gland.
Boster Scientific Support
Answered: 2020-05-06
Question
Our lab want to know about to test anti-LAR/PTPRF antibody PB9786 on human epithelium of mammary gland for research purposes, then I may be interested in using anti-LAR/PTPRF antibody PB9786 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-03-03
Answer
The products we sell, including anti-LAR/PTPRF antibody PB9786, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-03-03
Question
We are currently using anti-LAR/PTPRF antibody PB9786 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on horse tissues as well?
D. Zhao
Verified customer
Asked: 2019-10-29
Answer
The anti-LAR/PTPRF antibody (PB9786) has not been validated for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-29
Question
Is there a BSA free version of anti-LAR/PTPRF antibody PB9786 available?
Verified Customer
Verified customer
Asked: 2019-09-26
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-LAR/PTPRF antibody PB9786 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-09-26
Question
I was wanting to use your anti-LAR/PTPRF antibody for WB for human epithelium of mammary gland on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human epithelium of mammary gland identification?
Verified Customer
Verified customer
Asked: 2017-09-12
Answer
As indicated on the product datasheet, PB9786 anti-LAR/PTPRF antibody has been validated for IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human epithelium of mammary gland in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-09-12
