Product Info Summary
SKU: | A01753 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-SDHA Antibody Picoband®
SKU/Catalog Number
A01753
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-SDHA Antibody Picoband® catalog # A01753. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SDHA Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01753)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human SDHA, identical to the related mouse and rat sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01753 is reactive to SDHA in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
73 kDa
Calculated molecular weight
72.692kDa
Background of SDHA
Succinate dehydrogenase complex, subunit A, flavoprotein variant is a protein that in humans is encoded by the SDHA gene. It is mapped to 5p15.33. This gene encodes a major catalytic subunit of succinate-ubiquinone oxidoreductase, a complex of the mitochondrial respiratory chain. The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane. Mutations in this gene have been associated with a form of mitochondrial respiratory chain deficiency known as Leigh Syndrome. A pseudogene has been identified on chromosome 3q29. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01753 is guaranteed for Flow Cytometry, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.25μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human, Mouse, Rat
Positive Control
WB: human Hela whole cell, human HepG2 whole cell, human HEK293 whole cell, human Caco-2 whole cell, rat brain tissue, rat kidney tissue, rat spleen tissue, rat liver tissue, mouse brain tissue, mouse kidney tissue, mouse liver tissue
ICC/IF: Hela cell
FCM: Hela cell, RAW2647 cell, C6 cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. IF analysis of SDHA using anti-SDHA antibody (A01753).
SDHA was detected in immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-SDHA Antibody (A01753) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Click image to see more details
Figure 2. Western blot analysis of SDHA using anti-SDHA antibody (A01753).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human HEK293 whole cell lysates,
Lane 4: human Caco-2 whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat kidney tissue lysates,
Lane 7: rat spleen tissue lysates,
Lane 8: rat liver tissue lysates,
Lane 9: mouse brain tissue lysates,
Lane 10: mouse kidney tissue lysates,
Lane 11: mouse liver tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SDHA antigen affinity purified polyclonal antibody (Catalog # A01753) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SDHA at approximately 73KD. The expected band size for SDHA is at 73KD.
Click image to see more details
Figure 3. Flow Cytometry analysis of Hela cells using anti-SDHA antibody (A01753).
Overlay histogram showing Hela cells stained with A01753 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SDHA Antibody (A01753,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Click image to see more details
Figure 4. Flow Cytometry analysis of RAW264.7 cells using anti-SDHA antibody (A01753).
Overlay histogram showing RAW264.7 cells stained with A01753 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SDHA Antibody (A01753,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Click image to see more details
Figure 5. Flow Cytometry analysis of C6 cells using anti-SDHA antibody (A01753).
Overlay histogram showing C6 cells stained with A01753 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SDHA Antibody (A01753,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For SDHA (Source: Uniprot.org, NCBI)
Gene Name
SDHA
Full Name
Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial
Weight
72.692kDa
Superfamily
FAD-dependent oxidoreductase 2 family
Alternative Names
Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial; Flavoprotein subunit of complex II; Fp; SDHA; SDH2; SDHF SDHA CMD1GG, FP, MC2DN1, PGL5, SDH1, SDH2, SDHF succinate dehydrogenase complex flavoprotein subunit A succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial|flavoprotein subunit of complex II|succinate dehydrogenase complex, subunit A, flavoprotein (Fp)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SDHA, check out the SDHA Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SDHA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SDHA Antibody Picoband® (A01753)
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