Anti-LAG3 Antibody

Product Name

Anti-LAG3 Antibody

View all LAG-3 Antibodies

SKU/Catalog Number

A02869

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-LAG3 Antibody (Catalog # A02869). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse.

Storage & Handling

LAG3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-LAG3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02869)

Host

Rabbit

Contents

LAG3 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

LAG3 antibody was raised against a peptide corresponding to 18 amino acids near the amino terminus of human LAG3. The immunogen is located within amino acids 110 - 160 of LAG3.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

At least two antibodies are known to exist; this antibody will only detect the largest isoform.

Reactive Species

A02869 is reactive to LAG3 in Human, Mouse

Reconstitution

Observed Molecular Weight

68 kDa

Calculated molecular weight

57449 MW

Background of LAG-3

The lymphocyte activation gene-3 (LAG3) is a member of the immunoglobulin superfamily and binds MHC class II with high affinity (1), negatively regulating T-cell function and homeostasis (2). It is expressed in B, T, and NK cells, monocytes, and dendritic cells (3), and acts to regulate T cell expansion (4). LAG3 is also an important immune checkpoint protein, with anti-LAG3 antibodies activating T effector cells and affecting regulatory T cell functions. Furthermore LAG3 appears to act in a synergistic fashion with PD-1/PD-L1, suggesting that a dual antibody approach may prove useful in cancer immunotherapy (5).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A02869 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

LAG3 antibody can be used for Western blot at 1 - 2 μg/mL. Antibody can also be used for Immunohistochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

Gene/Protein Information For LAG3 (Source: Uniprot.org, NCBI)

Alternative Names

lymphocyte activating 3, LAG-3, CD223 LAG3 CD223 lymphocyte activating 3 lymphocyte activation gene 3 protein|lymphocyte-activation gene 3

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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