Anti-L1TD1 Antibody Picoband™

Product Name

Anti-L1TD1 Antibody Picoband™

View all L1TD1 Antibodies

SKU/Catalog Number

A12503-1

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-L1TD1 Antibody Picoband™ catalog # A12503-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-L1TD1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12503-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human L1TD1 recombinant protein (Position: D34-H852).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A12503-1 is reactive to L1TD1 in Human, Mouse, Rat

Applications

A12503-1 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

120 kDa

Calculated molecular weight

Background of L1TD1

L1TD1 is an RNA-binding protein highly and specifically expressed in pluripotent cells. It is essential for maintaining the pluripotentency in human embryonic stem cells(hESCs) and has been considered as a markerfor undifferentiated hESCs. L1TD1 is also reported to be highly expressed in medulloblastoma cells and its expression in medulloblastoma correlates with poor clinical outcome.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human, Mouse, Rat
ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of L1TD1 using anti-L1TD1 antibody (A12503-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Caco-2 whole cell lysates,
Lane 2: rat testis lysates,
Lane 3: rat C6 whole cell lysates,
Lane 4: rat PC-12 whole cell lysates,
Lane 5: mouse testis tissue lysates,
Lane 6: mouse NIH/3T3 whole cell lysates,
Lane 7: mouse RAW264.7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-L1TD1 antigen affinity purified polyclonal antibody (Catalog # A12503-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for L1TD1 at approximately 120 kDa. The expected band size for L1TD1 is at 120 kDa.

Gene/Protein Information For L1TD1 (Source: Uniprot.org, NCBI)

Alternative Names

ECAT11; ES cell-associated protein 11; FLJ10884; L1TD1; LINE-1 type transposase domain containing 1; LINE-1 type transposase domain-containing protein 1; MGC133253; RP5-1155K23.3 L1TD1 ECAT11 LINE1 type transposase domain containing 1 LINE-1 type transposase domain-containing protein 1|ES cell-associated protein 11|LINE-1 type transposase domain containing 1

For more info on L1TD1, check out the L1TD1 Infographic

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In this infographic, you will see the following information for L1TD1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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