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Product Info Summary
| SKU: | M00729 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-L1CAM Rabbit Monoclonal Antibody
SKU/Catalog Number
M00729
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-L1CAM Rabbit Monoclonal Antibody catalog # M00729. Tested in WB, IHC applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-L1CAM Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00729)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AAFD-12
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human L1CAM
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00729 is reactive to L1CAM in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
90 kDa
Calculated molecular weight
140003 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00729 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
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Western blot analysis of L1CAM expression in human fetal kidney lysate.
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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
Protein Target Info & Infographic
Gene/Protein Information For L1CAM (Source: Uniprot.org, NCBI)
Gene Name
L1CAM
Full Name
Neural cell adhesion molecule L1
Weight
140003 MW
Superfamily
immunoglobulin superfamily
Alternative Names
Neural cell adhesion molecule L1;N-CAM-L1;NCAM-L1;CD171;L1CAM;CAML1, MIC5; L1CAM CAML1, CD171, HSAS, HSAS1, MASA, MIC5, N-CAM-L1, N-CAML1, NCAM-L1, S10, SPG1 L1 cell adhesion molecule neural cell adhesion molecule L1| by R1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on L1CAM, check out the L1CAM Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for L1CAM: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-L1CAM Rabbit Monoclonal Antibody (M00729)
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No publications found for M00729
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Customer Reviews
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Customer Q&As
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14 Customer Q&As for Anti-L1CAM Rabbit Monoclonal Antibody
Question
Would M00729 anti-L1CAM Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-05-01
Answer
You can see on the product datasheet, M00729 anti-L1CAM Rabbit Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-05-01
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-L1CAM Rabbit Monoclonal antibody M00729. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-04-09
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-04-09
Question
I have attached the WB image, lot number and protocol we used for liver using anti-L1CAM Rabbit Monoclonal antibody M00729. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-04-08
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-04-08
Question
Is a blocking peptide available for product anti-L1CAM Rabbit Monoclonal antibody (M00729)?
N. Evans
Verified customer
Asked: 2020-03-18
Answer
We do provide the blocking peptide for product anti-L1CAM Rabbit Monoclonal antibody (M00729). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-03-18
Question
I was wanting to use your anti-L1CAM Rabbit Monoclonal antibody for WB for human liver on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human liver identification?
Verified Customer
Verified customer
Asked: 2019-06-05
Answer
It shows on the product datasheet, M00729 anti-L1CAM Rabbit Monoclonal antibody has been tested for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-06-05
Question
I am interested in to test anti-L1CAM Rabbit Monoclonal antibody M00729 on human liver for research purposes, then I may be interested in using anti-L1CAM Rabbit Monoclonal antibody M00729 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
K. Carter
Verified customer
Asked: 2018-04-23
Answer
The products we sell, including anti-L1CAM Rabbit Monoclonal antibody M00729, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-04-23
Question
I see that the anti-L1CAM Rabbit Monoclonal antibody M00729 works with WB, what is the protocol used to produce the result images on the product page?
G. Zhao
Verified customer
Asked: 2018-02-21
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-02-21
Question
My colleagues were happy with the WB result of your anti-L1CAM Rabbit Monoclonal antibody. However we have observed positive staining in cervix carcinoma cell membrane using this antibody. Is that expected? Could you tell me where is L1CAM supposed to be expressed?
Verified Customer
Verified customer
Asked: 2018-01-31
Answer
From literature, cervix carcinoma does express L1CAM. Generally L1CAM expresses in cell membrane. Regarding which tissues have L1CAM expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 20068231, 23186163
Fetal brain, Pubmed ID: 1769655, 1923824, 1932117
Liver, Pubmed ID: 19159218
Pancreas, Pubmed ID: 15489334
Plasma, Pubmed ID: 16335952
Boster Scientific Support
Answered: 2018-01-31
Question
Is this M00729 anti-L1CAM Rabbit Monoclonal antibody reactive to the isotypes of L1CAM?
M. Kulkarni
Verified customer
Asked: 2017-09-14
Answer
The immunogen of M00729 anti-L1CAM Rabbit Monoclonal antibody is A synthesized peptide derived from human L1CAM. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-09-14
Question
I have a question about product M00729, anti-L1CAM Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-07-17
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00729 anti-L1CAM Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-07-17
Question
Do you have a BSA free version of anti-L1CAM Rabbit Monoclonal antibody M00729 available?
Verified Customer
Verified customer
Asked: 2017-05-15
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-L1CAM Rabbit Monoclonal antibody M00729 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-05-15
Question
We are currently using anti-L1CAM Rabbit Monoclonal antibody M00729 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on canine tissues as well?
N. Li
Verified customer
Asked: 2016-12-20
Answer
The anti-L1CAM Rabbit Monoclonal antibody (M00729) has not been tested for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-12-20
Question
We have been able to see staining in human plasma. Any tips? Is anti-L1CAM Rabbit Monoclonal antibody supposed to stain plasma positively?
D. Zhang
Verified customer
Asked: 2016-07-26
Answer
Based on literature plasma does express L1CAM. Based on Uniprot.org, L1CAM is expressed in right hemisphere of cerebellum, fetal brain, pancreas, plasma, cervix carcinoma, liver, among other tissues. Regarding which tissues have L1CAM expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 20068231, 23186163
Fetal brain, Pubmed ID: 1769655, 1923824, 1932117
Liver, Pubmed ID: 19159218
Pancreas, Pubmed ID: 15489334
Plasma, Pubmed ID: 16335952
Boster Scientific Support
Answered: 2016-07-26
Question
Does anti-L1CAM Rabbit Monoclonal antibody M00729 work for WB with liver?
W. Thomas
Verified customer
Asked: 2016-04-05
Answer
According to the expression profile of liver, L1CAM is highly expressed in liver. So, it is likely that anti-L1CAM Rabbit Monoclonal antibody M00729 will work for WB with liver.
Boster Scientific Support
Answered: 2016-04-05
