Anti-CD62L/SELL Antibody Picoband®

L-Selectin/CD62L antibody

Boster Bio Anti-CD62L/SELL Antibody catalog # PA1359. Tested in WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance. Cited in 1 publication(s).

Product Info Summary

SKU: PA1359
Size: 100 μg/vial
Reactive Species: Mouse, Rat
Host: Rabbit
Application: WB

Product Name

Anti-CD62L/SELL Antibody Picoband®

View all L-Selectin/CD62L Antibodies

SKU/Catalog Number

PA1359

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-CD62L/SELL Antibody catalog # PA1359. Tested in WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CD62L/SELL Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1359)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of mouse L-selectin, identical to the related rat sequence.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1359 is reactive to Sell in Mouse, Rat

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

70 kDa

Calculated molecular weight

42288 MW

Background of L-Selectin/CD62L

L-selectin, also known as CD62L, is a cell adhesion molecule found on leukocytes. It belongs to the selectin family of proteins, which recognize sialylated carbohydrate groups. It is cleaved by ADAM17. SELL (L-selectin)is a cell surface component that is a member of a family of adhesion/homing receptors which play important roles in leukocyte-endothelial cell interactions. The molecule is composed of multiple domains: one homologous to lectins, one to epidermal growth factor, and two to the consensus repeat units found in C3/C4 binding proteins. L-selectin acts as a "homing receptor" for leukocytes to enter secondary lymphoid tissues via high endothelial venules. Ligands present on endothelial cells will bind to leukocyte expressing L-selectin, slowing leukocyte trafficking through the blood, and facilitating entry into a secondary lymphoid organ at that point.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

PA1359 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Mouse, Rat

Positive Control

WB: Rat Spleen Tissue, Mouse Spleen Tissue

Validation Images & Assay Conditions

Gene/Protein Information For Sell (Source: Uniprot.org, NCBI)

Gene Name

Sell

Full Name

L-selectin

Weight

42288 MW

Superfamily

selectin/LECAM family

Alternative Names

L-selectin;CD62 antigen-like family member L;Leukocyte adhesion molecule 1;LAM-1;Leukocyte-endothelial cell adhesion molecule 1;LECAM1;Lymph node homing receptor;Lymphocyte antigen 22;Ly-22;Lymphocyte surface MEL-14 antigen;CD62L;Sell;Lnhr, Ly-22, Ly22; SELL CD62L, LAM1, LECAM1, LEU8, LNHR, LSEL, LYAM1, PLNHR, TQ1 selectin L L-selectin|CD62 -like family member L|gp90-MEL|leukocyte surface Leu-8|leukocyte-endothelial cell adhesion molecule 1|lymph node homing receptor|lymphocyte adhesion molecule 1|pln homing receptor

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on Sell, check out the Sell Infographic

Sell infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for Sell: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

PA1359 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

HIV-1 Nef and Vpu Interfere with L-Selectin (CD62L) Cell Surface Expression To Inhibit Adhesion and Signaling in Infected CD4+ T Lymphocytes

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6 Customer Q&As for Anti-CD62L/SELL Antibody Picoband®

Question

Does anti-CD62L/SELL antibody PA1359 work for WB with hematopoietic?

Verified Customer

Verified customer

Asked: 2019-11-18

Answer

According to the expression profile of hematopoietic, SELL is highly expressed in hematopoietic. So, it is likely that anti-CD62L/SELL antibody PA1359 will work for WB with hematopoietic.

Boster Scientific Support

Answered: 2019-11-18

Question

Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for hematopoietic using anti-CD62L/SELL antibody PA1359. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-11-06

Answer

Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-11-06

Question

I was wanting to use your anti-CD62L/SELL antibody for WB for mouse hematopoietic on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse hematopoietic identification?

Verified Customer

Verified customer

Asked: 2019-10-16

Answer

You can see on the product datasheet, PA1359 anti-CD62L/SELL antibody has been tested for WB on mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse hematopoietic in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-10-16

Question

Do you have a BSA free version of anti-CD62L/SELL antibody PA1359 available?

Verified Customer

Verified customer

Asked: 2019-07-29

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CD62L/SELL antibody PA1359 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-07-29

Question

Would anti-CD62L/SELL antibody PA1359 work on canine WB with plasma?

N. Bhatt

Verified customer

Asked: 2017-12-01

Answer

Our lab technicians have not validated anti-CD62L/SELL antibody PA1359 on canine. You can run a BLAST between canine and the immunogen sequence of anti-CD62L/SELL antibody PA1359 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine plasma in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2017-12-01

Question

I would like to test anti-CD62L/SELL antibody PA1359 on mouse hematopoietic for research purposes, then I may be interested in using anti-CD62L/SELL antibody PA1359 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

J. Williams

Verified customer

Asked: 2015-03-04

Answer

The products we sell, including anti-CD62L/SELL antibody PA1359, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2015-03-04

Order DetailsPrice
PA1359

100μg

$370
PA1359-10ug

10μg sample (liquid)

$99
PA1359-Biotin

100 μg Biotin conjugated

$570
PA1359-Cy3

100 μg Cy3 conjugated

$570
PA1359-Dylight488

100 μg Dylight488 conjugated

$570
PA1359-Dylight550

100 μg Dylight550 conjugated

$570
PA1359-Dylight594

100 μg Dylight594 conjugated

$570
PA1359-FITC

100 μg FITC conjugated

$570
PA1359-HRP

100 μg HRP conjugated

$570
PA1359-APC

100 μg APC conjugated

$670
PA1359-PE

100 μg PE conjugated

$670
PA1359-iFluor647

100 μg iFluor647 conjugated

$670
PA1359-carrier-free

Carrier Free

$370

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PA1359
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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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