Product Info Summary
SKU: | PA1359 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Mouse, Rat |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-CD62L/SELL Antibody Picoband®
View all L-Selectin/CD62L Antibodies
SKU/Catalog Number
PA1359
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CD62L/SELL Antibody catalog # PA1359. Tested in WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD62L/SELL Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1359)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of mouse L-selectin, identical to the related rat sequence.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1359 is reactive to Sell in Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
70 kDa
Calculated molecular weight
42288 MW
Background of L-Selectin/CD62L
L-selectin, also known as CD62L, is a cell adhesion molecule found on leukocytes. It belongs to the selectin family of proteins, which recognize sialylated carbohydrate groups. It is cleaved by ADAM17. SELL (L-selectin)is a cell surface component that is a member of a family of adhesion/homing receptors which play important roles in leukocyte-endothelial cell interactions. The molecule is composed of multiple domains: one homologous to lectins, one to epidermal growth factor, and two to the consensus repeat units found in C3/C4 binding proteins. L-selectin acts as a "homing receptor" for leukocytes to enter secondary lymphoid tissues via high endothelial venules. Ligands present on endothelial cells will bind to leukocyte expressing L-selectin, slowing leukocyte trafficking through the blood, and facilitating entry into a secondary lymphoid organ at that point.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1359 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Mouse, Rat
Positive Control
WB: Rat Spleen Tissue, Mouse Spleen Tissue
Validation Images & Assay Conditions
Click image to see more details
Anti-L-selectin antibody, PA1359, Western blotting
All lanes: Anti L-selectin (PA1359) at 0.5ug/ml
Lane 1: Rat Spleen Tissue Lysate at 50ug
Lane 2: Mouse Spleen Tissue Lysate at 50ug
Predicted bind size: 42KD
Observed bind size: 70KD
Protein Target Info & Infographic
Gene/Protein Information For Sell (Source: Uniprot.org, NCBI)
Gene Name
Sell
Full Name
L-selectin
Weight
42288 MW
Superfamily
selectin/LECAM family
Alternative Names
L-selectin;CD62 antigen-like family member L;Leukocyte adhesion molecule 1;LAM-1;Leukocyte-endothelial cell adhesion molecule 1;LECAM1;Lymph node homing receptor;Lymphocyte antigen 22;Ly-22;Lymphocyte surface MEL-14 antigen;CD62L;Sell;Lnhr, Ly-22, Ly22; SELL CD62L, LAM1, LECAM1, LEU8, LNHR, LSEL, LYAM1, PLNHR, TQ1 selectin L L-selectin|CD62 -like family member L|gp90-MEL|leukocyte surface Leu-8|leukocyte-endothelial cell adhesion molecule 1|lymph node homing receptor|lymphocyte adhesion molecule 1|pln homing receptor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Sell, check out the Sell Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Sell: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD62L/SELL Antibody Picoband® (PA1359)
Hello CJ!
PA1359 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
HIV-1 Nef and Vpu Interfere with L-Selectin (CD62L) Cell Surface Expression To Inhibit Adhesion and Signaling in Infected CD4+ T Lymphocytes
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-CD62L/SELL Antibody Picoband®
Question
Does anti-CD62L/SELL antibody PA1359 work for WB with hematopoietic?
Verified Customer
Verified customer
Asked: 2019-11-18
Answer
According to the expression profile of hematopoietic, SELL is highly expressed in hematopoietic. So, it is likely that anti-CD62L/SELL antibody PA1359 will work for WB with hematopoietic.
Boster Scientific Support
Answered: 2019-11-18
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for hematopoietic using anti-CD62L/SELL antibody PA1359. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-11-06
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-06
Question
I was wanting to use your anti-CD62L/SELL antibody for WB for mouse hematopoietic on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse hematopoietic identification?
Verified Customer
Verified customer
Asked: 2019-10-16
Answer
You can see on the product datasheet, PA1359 anti-CD62L/SELL antibody has been tested for WB on mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse hematopoietic in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-16
Question
Do you have a BSA free version of anti-CD62L/SELL antibody PA1359 available?
Verified Customer
Verified customer
Asked: 2019-07-29
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CD62L/SELL antibody PA1359 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-07-29
Question
Would anti-CD62L/SELL antibody PA1359 work on canine WB with plasma?
N. Bhatt
Verified customer
Asked: 2017-12-01
Answer
Our lab technicians have not validated anti-CD62L/SELL antibody PA1359 on canine. You can run a BLAST between canine and the immunogen sequence of anti-CD62L/SELL antibody PA1359 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine plasma in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-12-01
Question
I would like to test anti-CD62L/SELL antibody PA1359 on mouse hematopoietic for research purposes, then I may be interested in using anti-CD62L/SELL antibody PA1359 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
J. Williams
Verified customer
Asked: 2015-03-04
Answer
The products we sell, including anti-CD62L/SELL antibody PA1359, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2015-03-04