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Product Info Summary
| SKU: | EKC2051 |
|---|---|
| Size: | 1 kit, containing two 96-well plates and all necessary reagents |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | Cell lines |
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Product info
Product Name
Ku80/XRCC5 (Phospho-Thr714) Colorimetric Cell-Based ELISA Kit
SKU/Catalog Number
EKC2051
Size
1 kit, containing two 96-well plates and all necessary reagents
Description
The Ku80/XRCC5 (Phospho-Thr714) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor Ku80/XRCC5 (Phospho-Thr714) protein expression profile in cells. The kit can be used for measuring the relative amounts of Ku80/XRCC5 (Phospho-Thr714) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on Ku80/XRCC5 (Phospho-Thr714).
Storage & Handling
Store at 4°C for up to 6 months.
Cite This Product
Ku80/XRCC5 (Phospho-Thr714) Colorimetric Cell-Based ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC2051)
Clonality of Antibodies
See Datasheet for details
Assay Range
> 5000 cells/well
Reactive Species
EKC2051 is reactive to XRCC5 in Human samples
Validated Sample Types
Cell lines
Application Guarantee
EKC2051 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Kit Components
| Reagent | Quantity | Container |
|---|---|---|
| 96-Well Cell Culture Clear-Bottom Microplate | 2 Plates | - |
| 10x TBS | 24 ml (10x) | Clear |
| Quenching Buffer | 24 ml (1x) | Clear |
| Blocking Buffer | 50 ml (1x) | Clear |
| 15x Wash Buffer | 50 ml (15x) | Clear |
| 100x Anti-Ku80/XRCC5 Phospho-Thr714 Antibody (Rabbit Polyclonal) | 60 µl (100x) | Red |
| 100x Anti-Ku80/XRCC5 Antibody (Rabbit Polyclonal) | 60 µl (100x) | Purple |
| 100x Anti-GAPDH Antibody (Mouse Monoclonal) | 60 µl (100x) | Green |
| HRP-Conjugated Anti-Rabbit IgG Antibody | 12ml (1x) | Glass |
| HRP-Conjugated Anti-Mouse IgG Antibody | 12ml (1x) | Glass |
| Primary Antibody Diluent | 12 ml (1x) | Clear |
| Ready-to-Use Substrate | 12 ml (1x) | Brown |
| Stop Solution | 12 ml (1x) | Clear |
| Crystal Violet Solution | 12ml (1x) | Glass |
| SDS Solution | 24 ml (1x) | Clear |
| Adhesive Plate Seals | 2 Seals | - |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
- Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
- 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
- Deionized or sterile water
- Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
- Graph paper or computer software capable of generating or displaying logarithmic functions
- Absorbent papers or vacuum aspirator
- Test tubes or microfuge tubes capable of storing 1 ml
- Orbital shaker
- Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)
Data Examples, Quality Control Data
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Boster Kit Box
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Western blot analysis of extracts from COS7 cells
Protein Target Info & Infographic
Gene/Protein Information For XRCC5 (Source: Uniprot.Org, NCBI)
Gene Name
XRCC5
Full Name
X-ray repair cross-complementing protein 5
Weight
82705 MW
Superfamily
ku80 family
Alternative Names
86 kDa subunit of Ku antigen;ATP-dependent DNA helicase II, 80 kDa subunit;CTC box binding factor 85 kDa subunit;CTC85;CTCBF;DNA-repair protein XRCC5;G22P2;KU86;Lupus Ku autoantigen protein p86;Nuclear factor IV;Thyroid- lupus autoantigen;TLAA;XRCC5 XRCC5 KARP-1, KARP1, KU80, KUB2, Ku86, NFIV X-ray repair cross complementing 5 X-ray repair cross-complementing protein 5|86 kDa subunit of Ku |ATP-dependent DNA helicase 2 subunit 2|ATP-dependent DNA helicase II 80 kDa subunit|CTC box-binding factor 85 kDa subunit|CTC85|CTCBF|DNA repair protein XRCC5|Ku auto, 80kDa|Ku86 auto related protein 1|TLAA|X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining)|lupus Ku auto protein p86|nuclear factor IV|thyroid-lupus auto
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on XRCC5, check out the XRCC5 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for XRCC5: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Ku80/XRCC5 (Phospho-Thr714) Colorimetric Cell-Based ELISA Kit (EKC2051)
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