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Product Info Summary
| SKU: | M00099-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, ICC, WB |
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Product info
Product Name
Anti-KRAS+HRAS+NRAS Rabbit Monoclonal Antibody
SKU/Catalog Number
M00099-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-KRAS+HRAS+NRAS Rabbit Monoclonal Antibody catalog # M00099-1. Tested in WB, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-KRAS+HRAS+NRAS Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00099-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
EBE-14
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human KRAS+HRAS+NRAS
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00099-1 is reactive to NRAS in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
21 kDa
Calculated molecular weight
21229 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00099-1 is guaranteed for Flow Cytometry, IP, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ICC/IF 1:50-1:200
IP 1:50
FC 1:200
Positive Control
WB: human 293T whole cell, human A431 whole cell, human Jurkat whole cell, human SW620 whole cell, rat PC-12 whole cell, rat C6 whole cell, mouse NIH/3T3 whole cell
ICC/IF: Hela cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of KRAS+HRAS+NRAS using anti-KRAS+HRAS+NRAS antibody (M00099-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human A431 whole cell lysates,
Lane 3: human Jurkat whole cell lysates,
Lane 4: human SW620 whole cell lysates,
Lane 5: rat PC-12 whole cell lysates,
Lane 6: rat C6 whole cell lysates,
Lane 7: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KRAS+HRAS+NRAS antigen affinity purified monoclonal antibody (Catalog # M00099-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KRAS+HRAS+NRAS at approximately 21 kDa. The expected band size for KRAS+HRAS+NRAS is at 21 kDa.
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Immunofluorescent analysis of Hela cells, using KRAS+HRAS+NRAS Antibody.
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KRAS+HRAS+NRAS was immunoprecipitated from 1mg of A549 whole cell lysate. A549 whole cell lysate 10ug (Input). The second antibody be without interference from denatured IgG.
Protein Target Info & Infographic
Gene/Protein Information For NRAS (Source: Uniprot.org, NCBI)
Gene Name
NRAS
Full Name
GTPase NRas
Weight
21229 MW
Superfamily
small GTPase superfamily
Alternative Names
GTPase NRas;Transforming protein N-Ras;NRAS;HRAS1; NRAS ALPS4, CMNS, N-ras, NCMS1, NS6, NRAS NRAS proto-oncogene, GTPase GTPase NRas|N-ras protein part 4|neuroblastoma RAS viral (v-ras) oncogene homolog|neuroblastoma RAS viral oncogene homolog|transforming protein N-Ras|v-ras neuroblastoma RAS viral oncogene homolog
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NRAS, check out the NRAS Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NRAS: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-KRAS+HRAS+NRAS Rabbit Monoclonal Antibody (M00099-1)
Hello CJ!
M00099-1 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Li M, Liu W, Zhu Yf, Chen Yl, Zhang Bz, Wang R. Acta Oncol. 2006;45(8):1115-9. Correlation Of Cox-2 And K-Ras Expression To Clinical Outcome In Gastric Cancer.
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Customer Q&As
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1 Customer Q&As for Anti-KRAS+HRAS+NRAS Rabbit Monoclonal Antibody
Question
We received M00099-1 warm and the blue ice has completely melted upon arrival. Could this still work?
Verified customer
Asked: 2020-11-30
Answer
For the Anti-KRAS+HRAS+NRAS Rabbit Monoclonal Antibody (M00099-1), you can run a pilot test to see if the antibody could work. We can provide a replacement if the antibody fails to work as expected.
Boster Scientific Support
Answered: 2020-12-01
