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Product Info Summary
| SKU: | A10086 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, WB |
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Product info
Product Name
Anti-KPB1/2 PHKA1 Antibody
SKU/Catalog Number
A10086
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-KPB1/2 PHKA1 Antibody (Catalog # A10086). Tested in WB, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-KPB1/2 PHKA1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10086)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human KPB1/2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A10086 is reactive to PHKA1 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
137312 MW
Background of PHKA1
Phosphorylase b kinase catalyzes the phosphorylation of serine in certain substrates, including troponin I. The alpha chain may bind calmodulin.
Wuellrich A., J. Biol. Chem. 268:23208-23214(1993).
The MGC Project Team, Genome Res. 14:2121-2127(2004).
Burwinkel B., Eur. J. Hum. Genet. 11:516-526(2003).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A10086 is guaranteed for IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Immunofluorescence: 1:100~1:500
Validation Images & Assay Conditions
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Western blot analysis of extracts from Jurkat cells and COLO205 cells, using KPB1/2 antibody A10086.
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Immunofluorescence analysis of HeLa cells, using KPB1/2 antibody A10086.
Protein Target Info & Infographic
Gene/Protein Information For PHKA1 (Source: Uniprot.org, NCBI)
Gene Name
PHKA1
Full Name
Phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform
Weight
137312 MW
Superfamily
phosphorylase b kinase regulatory chain family
Alternative Names
Phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform;Phosphorylase kinase alpha M subunit;PHKA1;PHKA; PHKA1 PHKA phosphorylase kinase regulatory subunit alpha 1 phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform|phosphorylase kinase alpha M subunit|phosphorylase kinase, alpha 1 (muscle), muscle glycogenosis
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PHKA1, check out the PHKA1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PHKA1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-KPB1/2 PHKA1 Antibody (A10086)
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No publications found for A10086
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Customer Reviews
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Customer Q&As
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3 Customer Q&As for Anti-KPB1/2 PHKA1 Antibody
Question
Will anti-KPB1/2 antibody A10086 work on canine IF with cervix carcinoma?
Verified Customer
Verified customer
Asked: 2020-03-30
Answer
Our lab technicians have not validated anti-KPB1/2 antibody A10086 on canine. You can run a BLAST between canine and the immunogen sequence of anti-KPB1/2 antibody A10086 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine cervix carcinoma in IF, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-03-30
Question
I am interested in to test anti-KPB1/2 antibody A10086 on mouse muscle for research purposes, then I may be interested in using anti-KPB1/2 antibody A10086 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-11-18
Answer
The products we sell, including anti-KPB1/2 antibody A10086, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-11-18
Question
We are currently using anti-KPB1/2 antibody A10086 for rat tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2019-08-22
Answer
The anti-KPB1/2 antibody (A10086) has not been tested for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-08-22
