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Product Info Summary
| SKU: | A08923 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-DDX54 Antibody
SKU/Catalog Number
A08923
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-DDX54 Antibody (Catalog # A08923). Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-DDX54 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08923)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human DDX54.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A08923 is reactive to DDX54 in Human
Reconstitution
Calculated molecular weight
98595 MW
Background of Ddx54
Has RNA-dependent ATPase activity. Represses the transcriptional activity of nuclear receptors.
Scherer S.E., Nature 440:346-351(2006) [PubMed: 16541075].
Rajendran R.R., J. Biol. Chem. 278:4628-4638(2003) [PubMed: 12466272].
Scherl A.,Mol. Biol. Cell 13:4100-4109(2002) [PubMed: 12429849].
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A08923 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
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Western blot analysis of extracts from CoLo cells, 293 cells and JurKat cells, using DDX54 antibody A08923.
Protein Target Info & Infographic
Gene/Protein Information For DDX54 (Source: Uniprot.org, NCBI)
Gene Name
DDX54
Full Name
ATP-dependent RNA helicase DDX54
Weight
98595 MW
Superfamily
DEAD box helicase family
Alternative Names
ATP-dependent RNA helicase DDX54;3.6.4.13;ATP-dependent RNA helicase DP97;DEAD box RNA helicase 97 kDa;DEAD box protein 54;DDX54;
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on DDX54, check out the DDX54 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for DDX54: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-DDX54 Antibody (A08923)
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No publications found for A08923
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-DDX54 Antibody
Question
Will anti-DDX54 antibody A08923 work on monkey WB with cervix carcinoma erythroleukemia?
Verified Customer
Verified customer
Asked: 2020-04-27
Answer
Our lab technicians have not validated anti-DDX54 antibody A08923 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-DDX54 antibody A08923 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey cervix carcinoma erythroleukemia in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-04-27
Question
Is a blocking peptide available for product anti-DDX54 antibody (A08923)?
Verified Customer
Verified customer
Asked: 2020-01-31
Answer
We do provide the blocking peptide for product anti-DDX54 antibody (A08923). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-01-31
Question
Is there a BSA free version of anti-DDX54 antibody A08923 available?
Verified Customer
Verified customer
Asked: 2020-01-15
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-DDX54 antibody A08923 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-01-15
Question
I see that the anti-DDX54 antibody A08923 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-09-17
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-09-17
Question
Would anti-DDX54 antibody A08923 work for WB with kidney?
Verified Customer
Verified customer
Asked: 2019-08-09
Answer
According to the expression profile of kidney, DDX54 is highly expressed in kidney. So, it is likely that anti-DDX54 antibody A08923 will work for WB with kidney.
Boster Scientific Support
Answered: 2019-08-09
Question
We are currently using anti-DDX54 antibody A08923 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2019-05-22
Answer
The anti-DDX54 antibody (A08923) has not been validated for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-05-22
Question
Is this A08923 anti-DDX54 antibody reactive to the isotypes of DDX54?
Verified Customer
Verified customer
Asked: 2019-05-03
Answer
The immunogen of A08923 anti-DDX54 antibody is Synthesized peptide derived from internal of human DDX54. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-05-03
