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Product Info Summary
| SKU: | A01731-1 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | IF, WB |
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Product info
Product Name
Anti-KNTC2 NDC80 Antibody
SKU/Catalog Number
A01731-1
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-KNTC2 NDC80 Antibody (Catalog # A01731-1). Tested in WB, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-KNTC2 NDC80 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01731-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human KNTC2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A01731-1 is reactive to NDC80 in Human, Mouse
Reconstitution
Calculated molecular weight
73913 MW
Background of NDC80
Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore.
Chen Y., Mol. Cell. Biol. 17:6049-6056(1997).
The MGC Project Team, Genome Res. 14:2121-2127(2004).
Zheng L., Mol. Cell. Biol. 19:5417-5428(1999).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01731-1 is guaranteed for IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Immunofluorescence: 1:100~1:500
Validation Images & Assay Conditions
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Western blot analysis of extracts from Jurkat cells, using KNTC2 antibody A01731-1.
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Immunofluorescence analysis of HUVEC cells, using KNTC2 antibody A01731-1.
Protein Target Info & Infographic
Gene/Protein Information For NDC80 (Source: Uniprot.org, NCBI)
Gene Name
NDC80
Full Name
Kinetochore protein NDC80 homolog
Weight
73913 MW
Superfamily
NDC80/HEC1 family
Alternative Names
Kinetochore protein NDC80 homolog;Highly expressed in cancer protein;Kinetochore protein Hec1;HsHec1;Kinetochore-associated protein 2;Retinoblastoma-associated protein HEC;NDC80;HEC, HEC1, KNTC2; NDC80 HEC, HEC1, HsHec1, KNTC2, TID3, hsNDC80 NDC80 kinetochore complex component kinetochore protein NDC80 homolog|NDC80 homolog, kinetochore complex component|NDC80 kinetochore complex component homolog|highly expressed in cancer protein|highly expressed in cancer, rich in leucine heptad repeats|kinetochore associated 2|kinetochore protein Hec1|kinetochore-associated protein 2|retinoblastoma-associated protein HEC
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NDC80, check out the NDC80 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NDC80: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-KNTC2 NDC80 Antibody (A01731-1)
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