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Product Info Summary
| SKU: | M00050-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-KMT6 / EZH2 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00050-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-KMT6 / EZH2 Rabbit Monoclonal Antibody catalog # M00050-1. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-KMT6 / EZH2 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00050-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
IDF-5
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human KMT6 / EZH2
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00050-1 is reactive to EZH2 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
45 kDa
Calculated molecular weight
85363 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00050-1 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:1000
IHC 1:50-1:200
ICC/IF 1:50-1:200
FC 1：50
Positive Control
WB: HEK293 cell
IHC: human tonsil tissue
ICC/IF: Hela cell
Validation Images & Assay Conditions
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Western blot analysis of KMT6 / EZH2 expression in HEK293 cell lysate.
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Immunohistochemical analysis of paraffin-embedded human tonsil, using KMT6 / EZH2 Antibody.
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Immunofluorescent analysis of Hela cells, using KMT6 / EZH2 Antibody.
Protein Target Info & Infographic
Gene/Protein Information For EZH2 (Source: Uniprot.org, NCBI)
Gene Name
EZH2
Full Name
Histone-lysine N-methyltransferase EZH2
Weight
85363 MW
Superfamily
class V-like SAM-binding methyltransferase superfamily
Alternative Names
Histone-lysine N-methyltransferase EZH2;2.1.1.43;ENX-1;Enhancer of zeste homolog 2;Lysine N-methyltransferase 6;EZH2;KMT6; EZH2 ENX-1, ENX1b, KMT6, KMT6A, WVS, WVS2, EZH2 enhancer of zeste 2 polycomb repressive complex 2 subunit histone-lysine N-methyltransferase EZH2|enhancer of zeste homolog 2|lysine N-methyltransferase 6
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on EZH2, check out the EZH2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for EZH2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-KMT6 / EZH2 Rabbit Monoclonal Antibody (M00050-1)
Hello CJ!
M00050-1 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Enhancer of zeste homolog 2 silencing inhibits tumor growth and lung metastasis in osteosarcoma
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Anti-KMT6 / EZH2 Rabbit Monoclonal Antibody
Question
Is this M00050-1 anti-KMT6 / EZH2 Rabbit Monoclonal antibody reactive to the isotypes of EZH2?
A. Wu
Verified customer
Asked: 2020-04-14
Answer
The immunogen of M00050-1 anti-KMT6 / EZH2 Rabbit Monoclonal antibody is A synthesized peptide derived from human KMT6 / EZH2. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-04-14
Question
Our team were content with the WB result of your anti-KMT6 / EZH2 Rabbit Monoclonal antibody. However we have been able to see positive staining in cervix carcinoma nucleus using this antibody. Is that expected? Could you tell me where is EZH2 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-03-17
Answer
Based on literature, cervix carcinoma does express EZH2. Generally EZH2 expresses in nucleus. Regarding which tissues have EZH2 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 8954776
Brain, and Testis, Pubmed ID: 14702039
Cervix carcinoma, Pubmed ID: 16964243, 17081983, 18220336, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Pancreas, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2020-03-17
Question
My question regarding product M00050-1, anti-KMT6 / EZH2 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-03-12
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00050-1 anti-KMT6 / EZH2 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-03-12
Question
Do you have a BSA free version of anti-KMT6 / EZH2 Rabbit Monoclonal antibody M00050-1 available?
Verified Customer
Verified customer
Asked: 2020-03-09
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-KMT6 / EZH2 Rabbit Monoclonal antibody M00050-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-03-09
Question
Will M00050-1 anti-KMT6 / EZH2 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-01-17
Answer
You can see on the product datasheet, M00050-1 anti-KMT6 / EZH2 Rabbit Monoclonal antibody as been tested on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-01-17
Question
We are currently using anti-KMT6 / EZH2 Rabbit Monoclonal antibody M00050-1 for human tissue, and we are happy with the ICC results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2019-10-09
Answer
The anti-KMT6 / EZH2 Rabbit Monoclonal antibody (M00050-1) has not been tested for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-09
Question
I see that the anti-KMT6 / EZH2 Rabbit Monoclonal antibody M00050-1 works with IF, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-05-20
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-05-20
Question
Is a blocking peptide available for product anti-KMT6 / EZH2 Rabbit Monoclonal antibody (M00050-1)?
Verified Customer
Verified customer
Asked: 2018-10-19
Answer
We do provide the blocking peptide for product anti-KMT6 / EZH2 Rabbit Monoclonal antibody (M00050-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-10-19
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lung using anti-KMT6 / EZH2 Rabbit Monoclonal antibody M00050-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-10-08
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-10-08
Question
I was wanting to use your anti-KMT6 / EZH2 Rabbit Monoclonal antibody for IF for human lung on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human lung identification?
Verified Customer
Verified customer
Asked: 2018-08-22
Answer
As indicated on the product datasheet, M00050-1 anti-KMT6 / EZH2 Rabbit Monoclonal antibody has been tested for Flow Cytometry, IF, IHC, ICC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human lung in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-08-22
Question
My lab would like to test anti-KMT6 / EZH2 Rabbit Monoclonal antibody M00050-1 on human lung for research purposes, then I may be interested in using anti-KMT6 / EZH2 Rabbit Monoclonal antibody M00050-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-12-01
Answer
The products we sell, including anti-KMT6 / EZH2 Rabbit Monoclonal antibody M00050-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-12-01
Question
Here is the WB image, lot number and protocol we used for lung using anti-KMT6 / EZH2 Rabbit Monoclonal antibody M00050-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2017-08-04
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-08-04
Question
Does anti-KMT6 / EZH2 Rabbit Monoclonal antibody M00050-1 work for IF with lung?
R. Evans
Verified customer
Asked: 2017-05-30
Answer
According to the expression profile of lung, EZH2 is highly expressed in lung. So, it is likely that anti-KMT6 / EZH2 Rabbit Monoclonal antibody M00050-1 will work for IF with lung.
Boster Scientific Support
Answered: 2017-05-30
Question
We ordered your anti-KMT6 / EZH2 Rabbit Monoclonal antibody for IF on cervix carcinoma erythroleukemia in the past. I am using human, and We intend to use the antibody for Flow Cytometry next. We need examining cervix carcinoma erythroleukemia as well as leukemic t-cell in our next experiment. Could you please give me some suggestion on which antibody would work the best for Flow Cytometry?
D. Krishna
Verified customer
Asked: 2016-08-16
Answer
I looked at the website and datasheets of our anti-KMT6 / EZH2 Rabbit Monoclonal antibody and it appears that M00050-1 has been tested on human in both IF and Flow Cytometry. Thus M00050-1 should work for your application. Our Boster satisfaction guarantee will cover this product for Flow Cytometry in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for Flow Cytometry detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2016-08-16
Question
We have seen staining in human pancreas. What should we do? Is anti-KMT6 / EZH2 Rabbit Monoclonal antibody supposed to stain pancreas positively?
A. Johnson
Verified customer
Asked: 2016-05-19
Answer
From what I have seen in literature pancreas does express EZH2. From what I have seen in Uniprot.org, EZH2 is expressed in lung, brain, brain testis, pancreas, cervix carcinoma, leukemic t-cell, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have EZH2 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 8954776
Brain, and Testis, Pubmed ID: 14702039
Cervix carcinoma, Pubmed ID: 16964243, 17081983, 18220336, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Pancreas, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2016-05-19
