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Product Info Summary
| SKU: | A12497 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | IF, IHC, WB |
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Product info
Product Name
Anti-KLHL22 Monoclonal Antibody
SKU/Catalog Number
A12497
Size
100μl
Form
Liquid
Description
Boster Bio Anti-KLHL22 Monoclonal Antibody catalog # A12497. Tested in IF, IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-KLHL22 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12497)
Host
Mouse
Contents
Ascitic fluid containing 0.03% sodium azide.
Clonality
Monoclonal
Clone Number
7B1
Isotype
IgG
Immunogen
Purified recombinant fragment of human KLHL22 expressed in E. Coli.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A12497 is reactive to KLHL22 in Human
Reconstitution
Calculated molecular weight
71667 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A12497 is guaranteed for IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
IF, 1:200-1:1000
Validation Images & Assay Conditions
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Western Blot (WB) analysis using KLHL22 Monoclonal antibody against Mouse Brain tissues lysate.
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Immunofluorescence (IF) analysis of U251 cells using KLHL22 Monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Protein Target Info & Infographic
Gene/Protein Information For KLHL22 (Source: Uniprot.org, NCBI)
Gene Name
KLHL22
Full Name
Kelch-like protein 22
Weight
71667 MW
Alternative Names
Kelch-like protein 22;KLHL22; KLHL22 KELCHL kelch like family member 22 kelch-like protein 22|kelch-like 22
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on KLHL22, check out the KLHL22 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for KLHL22: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-KLHL22 Monoclonal Antibody (A12497)
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No publications found for A12497
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Customer Reviews
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Customer Q&As
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3 Customer Q&As for Anti-KLHL22 Monoclonal Antibody
Question
We are currently using anti-KLHL22 Monoclonal antibody A12497 for human tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on horse tissues as well?
D. Patel
Verified customer
Asked: 2019-12-23
Answer
The anti-KLHL22 Monoclonal antibody (A12497) has not been validated for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-23
Question
I was wanting to use your anti-KLHL22 Monoclonal antibody for WB for human liver on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human liver identification?
S. Edwards
Verified customer
Asked: 2018-01-12
Answer
You can see on the product datasheet, A12497 anti-KLHL22 Monoclonal antibody has been validated for IF, IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-01-12
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-KLHL22 Monoclonal antibody A12497. Let me know if you need anything else.
M. Moore
Verified customer
Asked: 2014-11-17
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-11-17
