Kelch-like protein 22 Antibodies

AND KLHL22 ELISA kits, Kelch-like protein 22 Proteins

Many biological assays use antibodies to detect Kelch-like protein 22, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with Kelch-like protein 22 in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop Kelch-like protein 22 antibodies...

We validate the specificity of these antibodies to Kelch-like protein 22 by testing them on tissues known to express KLHL22 positively and negatively. Browse below to find the KLHL22 antibody that suites your experiment. We have 3 of these antibodies and many publications and validation images.

If you cannot find antibodies that fit your needs, contact us for making custom antibodies. We have a full suite of custom antibody services covering from research to diagnostic and therapeutic applications.

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KLHL22 Infographic by Boster Bio

All KLHL22 Products

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Product

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Specificity

Applications

Price

Anti-KLHL22 Monoclonal Antibody

3 Q&As

Human

IF, IHC, WB

$399
Cat # A12497
100μl

KLHL22 (NM_032775) Human Recombinant Protein

$813
Cat # PROTQ53GT1
20 µg

KLHL22 (NM_032775) Human Over-expression Lysate

Human

$628
Cat # LS084861
100 µg

KLHL22 Overview

Facts about Kelch-like protein 22.

KLHL22 3D structure. Source: alphafold

Kelch-like protein 22 (KLHL22)

Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex required for chromosome alignment and localization of PLK1 in kinetochores. The BCR(KLHL22) ubiquitin ligase complex mediates monoubiquitination of PLK1, leading to PLK1 dissociation from phosphoreceptor proteins and subsequent removal from kinetochores, allowing silencing of the spindle assembly checkpoint (SAC) and chromosome segregation.

Monoubiquitination of PLK1 does not lead to PLK1 degradation. .

Gene Information

Human
Gene Name:	KLHL22
Uniprot:	Q53GT1
Entrez:	84861

Family

Belongs to:

No superfamily

Alternative Names

FLJ14360; KELCHL; kelch-like 22 (Drosophila); kelch-like protein 22

Molecular Weight

Mass (kDA):
71.667 kDA

Genomic Context


Human

Location:	22q11.21
Sequence:	22; NC_000022.11 (20441519..20497305, complement)

Subcellular Localizations

Cytoplasm, cytosol. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle. Nucleus. Lysosome. Mainly cytoplasmic in prophase and prometaphase. Associates with the mitotic spindle as the cells reach chromosome bi-orientation. Localizes to the centrosomes shortly before cells enter anaphase After anaphase onset, predominantly associates with the polar microtubules connecting the 2 opposing centrosomes and gradually diffuses into the cytoplasm during telophase (PubMed:23455478). Localizes to the nucleus upon amino acid starvation (PubMed:29769719). R

Interacting Proteins

NUDCD3

Pathways

Mitosis
Anaphase
Cytokinesis

PTMs

Ubiquitination

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/KLHL22

References

PMID: 19995937 by Maerki S., et al. The Cul3-KLHL21 E3 ubiquitin ligase targets aurora B to midzone microtubules in anaphase and is required for cytokinesis.

PMID: 24067371 by Metzger T., et al. CUL3 and protein kinases: insights from PLK1/KLHL22 interaction.