Anti-KIF22 Antibody (Center)

Product Name

Anti-KIF22 Antibody (Center)

View all KIF22 Antibodies

SKU/Catalog Number

M05570

Size

80 µl

Description

Boster Bio Anti-KIF22 Antibody (Center) (Catalog # M05570). Tested in WB, IHC-P, IF application(s). This antibody reacts with Human.

Storage & Handling

Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Cite This Product

Anti-KIF22 Antibody (Center) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05570)

Host

Rabbit

Contents

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

This KIF22 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 423-456 amino acids from the Central region of human KIF22.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M05570 is reactive to KIF22 in Human

Reconstitution

Observed Molecular Weight

Calculated molecular weight

73262 Da

Background of KIF22

Kinesin family that is involved in spindle formation and the movements of chromosomes during mitosis and meiosis. Binds to microtubules and to DNA.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M05570 is guaranteed for IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

IF: 1:25
WB: 1:2000
IHC-P: 1:25

Positive Control

Validation Images & Assay Conditions

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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF-7 (human breast cancer cell line) cells labeling KIF22 with M05570 at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm and nucleus staining on MCF-7 cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin at 1/100 dilution (red).The nuclear counter stain is DAPI (blue).

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All lanes : Anti-KIF22 Antibody (Center) at 1:2000 dilution
Lane 1: MCF-7 whole cell lysates
Lane 2: Jurkat whole cell lysates
Lane 3: SH-SY5Y whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size : 73 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.

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All lanes : Anti-KIF22 Antibody (Center) at 1:2000 dilution
Lane 1: MCF-7 whole cell lysates
Lane 2: Hela whole cell lysates
Lane 3: SH-SY5Y whole cell lysates
Lane 4: Jurkat whole cell lysates
Lane 5: HL-60 whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size : 73 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.

Click image to see more details

M05570 staining KIF22 in human skin tissue sections by Immunohistochemistry (IHC-P -paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

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M05570 staining KIF22 in human kidney tissue sections by Immunohistochemistry (IHC-P -paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Gene/Protein Information For KIF22 (Source: Uniprot.org, NCBI)

Alternative Names

Kid; KIDA-328A3.2; kinesin family member 22; kinesin-like 4; Kinesin-like DNA-binding protein; Kinesin-like protein 4; kinesin-like protein KIF22; KNSL4kinesin-like DNA-binding protein pseudogene; OBP; OBP-1; OBP-2; origin of plasmid DNA replication-binding protein; oriP binding protein KIF22 A-328A3.2, KID, KNSL4, OBP, OBP-1, OBP-2, SEMDJL2 kinesin family member 22 kinesin-like protein KIF22|kinesin-like DNA-binding protein pseudogene|kinesin-like protein 4|oriP binding protein|origin of plasmid DNA replication-binding protein

For more info on KIF22, check out the KIF22 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for KIF22: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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