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Product Info Summary
| SKU: | A00745 |
|---|---|
| Size: | 100uL |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Cyclin B1 CCNB1 Antibody
SKU/Catalog Number
A00745
Size
100uL
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-Cyclin B1 CCNB1 Antibody (Catalog # A00745). Tested in IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)
Cite This Product
Anti-Cyclin B1 CCNB1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00745)
Host
Rabbit
Contents
0.01% (w/v) Sodium Azide
Clonality
Polyclonal
Isotype
Antiserum
Immunogen
Anti-Cyclin B1 antibody was produced by repeated immunizations of full length fusion protein corresponding to the human gene.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A00745 is reactive to CCNB1 in Human
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
48337 MW
Background of Cyclin B1
The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product forms a complex with p34 (cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00745 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:2,000 - 1:10,000
IHC: 1:500
IP: 1:100
WB: 1:500 - 1:1,000
Validation Images & Assay Conditions
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Western blot analysis of Cyclin B1 expression in asynchronous HN30 cell lysates. Cyclin B1 at 62KD was detected using rabbit anti-Cyclin B1 polyclonal antibody (Catalog # A00745) at 1:500. Personal communication, Luca Cote, Temple University, Philadelphia, PA.
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Western blot analysis of Cyclin B1 expression in asynchronous HeLa cell lysates. Cyclin B1 at 55KD was detected using rabbit anti-Cyclin B1 polyclonal antibody (Catalog # A00745) at 1:500. Personal communication, Luca D'Agostino, Temple University, Philadelphia, PA.
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Cyclin B1 was detected in paraffin-embedded sections of human brain cerebellum tissue using rabbit anti-Cyclin B1 polyclonal antibody (Catalog # A00745). The immunohistochemical section was developed using SABC method (Catalog # SA1022). The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.
Protein Target Info & Infographic
Gene/Protein Information For CCNB1 (Source: Uniprot.org, NCBI)
Gene Name
CCNB1
Full Name
G2/mitotic-specific cyclin-B1
Weight
48337 MW
Superfamily
cyclin family
Alternative Names
cyclin B1, G2/mitotic-specific cyclin-B1 CCNB1 CCNB cyclin B1 G2/mitotic-specific cyclin-B1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CCNB1, check out the CCNB1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CCNB1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Cyclin B1 CCNB1 Antibody (A00745)
Hello CJ!
A00745 has been cited in 4 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Wang J,Wang L,Chen S,Peng H,Xiao L,E Du,Liu Y,Lin D,Wang Y,Xu Y,Yang K.PKMYT1 is associated with prostate cancer malignancy and may serve as a therapeutic target.Gene.2020 Jun 20;744:144608.doi:10.1016/j. gene.2020.144608.Epub 2020 Mar 29.PMID:32234541.
Species: Human
A00745 usage in article: APP:WB, SAMPLE:LNCAP CELL,C4-2 CELL,PC-3 CELL AND DU 145 CELL, DILUTION:1:200
mTORC1 regulates PTHrP to coordinate chondrocyte growth, proliferation and differentiation
Li K, Zhu Zc, Liu Yj, Liu Jw, Wang Ht, Xiong Zq, Shen X, Hu Zl, Zheng J. Int J Clin Exp Pathol. 2013 Sep 15;6(11):2460-7. Ecollection 2013. Zfx Knockdown Inhibits Growth And Migration Of Non-Small Cell Lung Carcinoma Cell Line H1299.
Yang H, Gu J, Zheng Q, Li M, Lian X, Miao J, Jiang J, Wei W. J Biol Chem. 2011 Apr 1;286(13):11865-74. Doi: 10.1074/Jbc.M110.136929. Epub 2011 Feb 10. Rpb5-Mediating Protein Is Required For The Proliferation Of Hepatocellular Carcinoma Cells.
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