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Product Info Summary
| SKU: | PB9762 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Keap1 Antibody Picoband®
SKU/Catalog Number
PB9762
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Keap1 Antibody Picoband® catalog # PB9762. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Keap1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9762)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Keap1 recombinant protein (Position: E25-E205). Human Keap1 shares 96.7% and 97.2% amino acid (aa) sequence identity with mouse and rat Keap1, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB9762 is reactive to KEAP1 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
66 kDa
Calculated molecular weight
69666 MW
Background of KEAP1
KEAP1 (KELCH-LIKE ECH-ASSOCIATED PROTEIN 1), is a protein that in humans is encoded by the Keap1 gene. The KIAA0132 gene is mapped on 19p13.2. Keap1 contains a central BTB/POZ domain and a C-terminal double glycine repeat (DGR), or Kelch, module. Keap1 has been shown to interact with Nrf2, a master regulator of the antioxidant response, which is important for the amelioration of oxidative stress. In the presence of the electrophilic agent diethylmalate, Nrf2 activity is released from Keap1 and Nrf2 translocate to the nucleus. Under quiescent conditions, Nrf2 is anchored in the cytoplasm through binding to Keap1, which, in turn, facilitates the ubiquitination and subsequent proteolysis of Nrf2. Because Nrf2 activation leads to a coordinated antioxidant and anti-inflammatory response, and Keap1 represses Nrf2 activation, Keap1 has become a very attractive drug target.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9762 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: human Hela whole cell, human MCF-7 whole cell, human HepG2 whole cell, human A549 whole cell, human PC-3 whole cell, human Caco-2 whole cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of Keap1 using anti-Keap1 antibody (PB9762).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human MCF-7 whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human A549 whole cell lysates,
Lane 5: human PC-3 whole cell lysates,
Lane 6: human Caco-2 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Keap1 antigen affinity purified polyclonal antibody (Catalog # PB9762) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Keap1 at approximately 66 kDa. The expected band size for Keap1 is at 70 kDa.
Protein Target Info & Infographic
Gene/Protein Information For KEAP1 (Source: Uniprot.org, NCBI)
Gene Name
KEAP1
Full Name
Kelch-like ECH-associated protein 1
Weight
69666 MW
Superfamily
KEAP1 family
Alternative Names
Kelch-like ECH-associated protein 1;Cytosolic inhibitor of Nrf2;INrf2;Kelch-like protein 19;KEAP1;INRF2, KIAA0132, KLHL19; KEAP1 INrf2, KLHL19 kelch like ECH associated protein 1 kelch-like ECH-associated protein 1|KEAP1 delta C|cytosolic inhibitor of Nrf2|kelch-like family member 19|kelch-like protein 19
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on KEAP1, check out the KEAP1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for KEAP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Keap1 Antibody Picoband® (PB9762)
Hello CJ!
PB9762 has been cited in 3 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Bone marrow mesenchymal stem cells protect against bleomycin-induced pulmonary fibrosis in rat by activating Nrf2 signaling
Zhu X, Chen Y, Chen Q, Yang H, Xie X. J Diabetes Res. 2018 Mar 21;2018:6730315. doi: 10.1155/2018/6730315. eCollection 2018. Astaxanthin Promotes Nrf2/ARE Signaling to Alleviate Renal Fibronectin and Collagen IV Accumulation in Diabetic Rats
Deng Y, Zhu J, Mi C, Xu B, Jiao C, Li Y, Xu D, Liu W, Xu Z. Neurotox Res. 2015 Feb;27(2):156-71. Doi: 10.1007/S12640-014-9489-5. Epub 2014 Oct 7. Melatonin Antagonizes Mn-Induced Oxidative Injury Through The Activation Of Keap1-Nrf2-Are Signaling ...
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Customer Q&As
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16 Customer Q&As for Anti-Keap1 Antibody Picoband®
Question
you antibody to test anti-Keap1 antibody PB9762 on human bone marrow for research purposes, then I may be interested in using anti-Keap1 antibody PB9762 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-02-24
Answer
The products we sell, including anti-Keap1 antibody PB9762, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-02-24
Question
We have seen staining in human bone marrow. Any tips? Is anti-Keap1 antibody supposed to stain bone marrow positively?
V. Banerjee
Verified customer
Asked: 2019-11-14
Answer
From literature bone marrow does express KEAP1. From Uniprot.org, KEAP1 is expressed in muscle of leg, bone marrow, brain, skin uterus, among other tissues. Regarding which tissues have KEAP1 expression, here are a few articles citing expression in various tissues:
Bone marrow, Pubmed ID: 8590280
Brain, Skin, and Uterus, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-11-14
Question
We are currently using anti-Keap1 antibody PB9762 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2019-10-18
Answer
The anti-Keap1 antibody (PB9762) has not been tested for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-18
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for bone marrow using anti-Keap1 antibody PB9762. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-01-21
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-01-21
Question
Can you help my question with product PB9762, anti-Keap1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-12-04
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9762 anti-Keap1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-12-04
Question
My boss were happy with the WB result of your anti-Keap1 antibody. However we have observed positive staining in bone marrow cytoplasm using this antibody. Is that expected? Could you tell me where is KEAP1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2018-05-01
Answer
Based on literature, bone marrow does express KEAP1. Generally KEAP1 expresses in cytoplasm. Regarding which tissues have KEAP1 expression, here are a few articles citing expression in various tissues:
Bone marrow, Pubmed ID: 8590280
Brain, Skin, and Uterus, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2018-05-01
Question
Does PB9762 anti-Keap1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
D. Miller
Verified customer
Asked: 2018-04-05
Answer
You can see on the product datasheet, PB9762 anti-Keap1 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-04-05
Question
Would anti-Keap1 antibody PB9762 work on goat WB with bone marrow?
Verified Customer
Verified customer
Asked: 2018-02-13
Answer
Our lab technicians have not validated anti-Keap1 antibody PB9762 on goat. You can run a BLAST between goat and the immunogen sequence of anti-Keap1 antibody PB9762 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat bone marrow in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-02-13
Question
See below the WB image, lot number and protocol we used for bone marrow using anti-Keap1 antibody PB9762. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-01-09
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-01-09
Question
I see that the anti-Keap1 antibody PB9762 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2017-11-24
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-11-24
Question
Is this PB9762 anti-Keap1 antibody reactive to the isotypes of KEAP1?
E. Kulkarni
Verified customer
Asked: 2017-02-15
Answer
The immunogen of PB9762 anti-Keap1 antibody is E.coli-derived human Keap1 recombinant protein (Position: E25-E205). Human Keap1 shares 96.7% and 97.2% amino acid (aa) sequence identity with mouse and rat Keap1, respectively. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-02-15
Question
I am interested in using your anti-Keap1 antibody for regulation of epidermal cell differentiation studies. Has this antibody been tested with western blotting on sw620 whole cell lysate? We would like to see some validation images before ordering.
L. Singh
Verified customer
Asked: 2017-01-23
Answer
I appreciate your inquiry. This PB9762 anti-Keap1 antibody is tested on hela whole cell lysate, sw620 whole cell lysate. It is guaranteed to work for WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2017-01-23
Question
Do you have a BSA free version of anti-Keap1 antibody PB9762 available?
V. Taylor
Verified customer
Asked: 2016-10-03
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Keap1 antibody PB9762 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2016-10-03
Question
Does anti-Keap1 antibody PB9762 work for WB with bone marrow?
C. Jones
Verified customer
Asked: 2016-06-21
Answer
According to the expression profile of bone marrow, KEAP1 is highly expressed in bone marrow. So, it is likely that anti-Keap1 antibody PB9762 will work for WB with bone marrow.
Boster Scientific Support
Answered: 2016-06-21
Question
Is a blocking peptide available for product anti-Keap1 antibody (PB9762)?
D. Yang
Verified customer
Asked: 2016-04-18
Answer
We do provide the blocking peptide for product anti-Keap1 antibody (PB9762). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2016-04-18
Question
I was wanting to use your anti-Keap1 antibody for WB for human bone marrow on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human bone marrow identification?
D. Anderson
Verified customer
Asked: 2013-05-23
Answer
It shows on the product datasheet, PB9762 anti-Keap1 antibody has been validated for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human bone marrow in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-05-23
