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Product Info Summary
| SKU: | A04502 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-KCNK1/Twik 1 Antibody
SKU/Catalog Number
A04502
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-KCNK1/Twik 1 Antibody (Catalog # A04502). Tested in WB applications. This antibody reacts with Human, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-KCNK1/Twik 1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04502)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human KCNK1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A04502 is reactive to KCNK1 in Human, Rat
Reconstitution
Calculated molecular weight
38143 MW
Background of KCNK1
Weakly inward rectifying potassium channel.
Lesage F., EMBO J. 15:1004-1011(1996).
Goldstein S.A.N., J. Mol. Med. 76:13-20(1998).
Orias M., Am. J. Physiol. 273:F663-F666(1997)
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04502 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
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Western blot analysis of extracts from Jurkat cells, using KCNK1 antibody A04502.
Protein Target Info & Infographic
Gene/Protein Information For KCNK1 (Source: Uniprot.org, NCBI)
Gene Name
KCNK1
Full Name
Potassium channel subfamily K member 1
Weight
38143 MW
Superfamily
two pore domain potassium channel (TC 1.A.1.8) family
Alternative Names
Potassium channel subfamily K member 1;Inward rectifying potassium channel protein TWIK-1 ;Potassium channel K2P1 ;Potassium channel KCNO1;KCNK1;HOHO1 , KCNO1, TWIK1; KCNK1 DPK, HOHO, K2P1, K2p1.1, KCNO1, TWIK-1, TWIK1 potassium two pore domain channel subfamily K member 1 potassium channel subfamily K member 1|inward rectifying potassium channel protein TWIK-1|potassium channel K2P1|potassium channel KCNO1|potassium channel, two pore domain subfamily K, member 1|potassium inwardly-rectifying channel, subfamily K, member 1|tandem of P domains in a weak inward rectifying K+ channel 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on KCNK1, check out the KCNK1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for KCNK1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-KCNK1/Twik 1 Antibody (A04502)
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No publications found for A04502
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Customer Q&As
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13 Customer Q&As for Anti-KCNK1/Twik 1 Antibody
Question
Would A04502 anti-KCNK1/Twik 1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-04-10
Answer
You can see on the product datasheet, A04502 anti-KCNK1/Twik 1 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-04-10
Question
Do you have a BSA free version of anti-KCNK1/Twik 1 antibody A04502 available?
Verified Customer
Verified customer
Asked: 2019-09-19
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-KCNK1/Twik 1 antibody A04502 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-09-19
Question
Is this A04502 anti-KCNK1/Twik 1 antibody reactive to the isotypes of KCNK1?
Verified Customer
Verified customer
Asked: 2019-08-21
Answer
The immunogen of A04502 anti-KCNK1/Twik 1 antibody is Synthesized peptide derived from internal of human KCNK1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-08-21
Question
I was wanting to use your anti-KCNK1/Twik 1 antibody for WB for rat kidney on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat kidney identification?
Verified Customer
Verified customer
Asked: 2019-07-08
Answer
You can see on the product datasheet, A04502 anti-KCNK1/Twik 1 antibody has been validated for WB on human, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat kidney in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-07-08
Question
I see that the anti-KCNK1/Twik 1 antibody A04502 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-06-28
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-06-28
Question
See attached the WB image, lot number and protocol we used for kidney using anti-KCNK1/Twik 1 antibody A04502. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-06-03
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-03
Question
My question regarding product A04502, anti-KCNK1/Twik 1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
D. Jones
Verified customer
Asked: 2019-06-03
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04502 anti-KCNK1/Twik 1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-06-03
Question
Will anti-KCNK1/Twik 1 antibody A04502 work for WB with kidney?
M. Kulkarni
Verified customer
Asked: 2018-11-14
Answer
According to the expression profile of kidney, KCNK1 is highly expressed in kidney. So, it is likely that anti-KCNK1/Twik 1 antibody A04502 will work for WB with kidney.
Boster Scientific Support
Answered: 2018-11-14
Question
Will anti-KCNK1/Twik 1 antibody A04502 work on dog WB with brain?
Verified Customer
Verified customer
Asked: 2018-05-23
Answer
Our lab technicians have not validated anti-KCNK1/Twik 1 antibody A04502 on dog. You can run a BLAST between dog and the immunogen sequence of anti-KCNK1/Twik 1 antibody A04502 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated dog samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in dog brain in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-05-23
Question
Is a blocking peptide available for product anti-KCNK1/Twik 1 antibody (A04502)?
Verified Customer
Verified customer
Asked: 2017-11-24
Answer
We do provide the blocking peptide for product anti-KCNK1/Twik 1 antibody (A04502). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-11-24
Question
We are currently using anti-KCNK1/Twik 1 antibody A04502 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, rat. Is it possible that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2017-10-06
Answer
The anti-KCNK1/Twik 1 antibody (A04502) has not been validated for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-10-06
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for kidney using anti-KCNK1/Twik 1 antibody A04502. Let me know if you need anything else.
C. Wu
Verified customer
Asked: 2016-10-21
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-10-21
Question
I am interested in to test anti-KCNK1/Twik 1 antibody A04502 on rat kidney for research purposes, then I may be interested in using anti-KCNK1/Twik 1 antibody A04502 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
G. Miller
Verified customer
Asked: 2016-06-08
Answer
The products we sell, including anti-KCNK1/Twik 1 antibody A04502, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2016-06-08
