Product Info Summary
SKU: | M05470 |
---|---|
Size: | 100ug/vial |
Reactive Species: | Human |
Host: | Mouse |
Application: | Flow Cytometry, IHC |
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Product info
Product Name
Anti-Kappa Light Chain (B-Cell Marker) IGKV1D-16 Monoclonal Antibody
View all Kappa Light Chain Antibodies
SKU/Catalog Number
M05470
Size
100ug/vial
Form
Liquid
Description
Boster Bio Anti-Kappa Light Chain (B-Cell Marker) IGKV1D-16 Monoclonal Antibody (Catalog # M05470). Tested in Flow Cytometry, IHC applications. This antibody reacts with Human.
Storage & Handling
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Cite This Product
Anti-Kappa Light Chain (B-Cell Marker) IGKV1D-16 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05470)
Host
Mouse
Contents
Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Clonality
Monoclonal
Clone Number
Clone: SPM508
Isotype
IgG1, kappa
Immunogen
Human B-Lymphoma Cells
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Does not cross-react with primate, avian or amphibian GR.
Reactive Species
M05470 is reactive to IGKC in Human
Applications
M05470 is guaranteed for Flow Cytometry, IHC Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
12730 MW
Background of Kappa Light Chain
This monoclonal antibody is specific to kappa light chain of immunoglobulin and shows no cross-reaction with lambda light chain or any of the five heavy chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. The ratio of kappa to lambda is 70:30. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the kappa light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Reconstitute with distilled water.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow Cytometry (0.5-2.0ug/million cells)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.
Validation Images & Assay Conditions
Click image to see more details
Formalin-fixed, paraffin-embedded human Tonsil stained with Anti-Kappa Light Chain Monoclonal Antibody (SPM508).
Protein Target Info & Infographic
Gene/Protein Information For IGKC (Source: Uniprot.org, NCBI)
Gene Name
IGKC
Full Name
Immunoglobulin kappa constant
Weight
12730 MW
Alternative Names
HCAK1; IGKCD; immunoglobulin kappa constant; Km; MGC111575; MGC62011; MGC72072; MGC88770; MGC88771; MGC88809 IGKC HCAK1D, Km, IGKC immunoglobulin kappa constant immunoglobulin kappa (invariant region)|immunoglobulin kappa constant region|immunoglobulin kappa light chain (VJ)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IGKC, check out the IGKC Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IGKC: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Kappa Light Chain (B-Cell Marker) IGKV1D-16 Monoclonal Antibody (M05470)
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Customer Reviews
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Customer Q&As
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3 Customer Q&As for Anti-Kappa Light Chain (B-Cell Marker) IGKV1D-16 Monoclonal Antibody
Question
Is a blocking peptide available for product anti-Kappa Light Chain (B-Cell Marker) Monoclonal antibody (M05470)?
Verified Customer
Verified customer
Asked: 2019-07-19
Answer
We do provide the blocking peptide for product anti-Kappa Light Chain (B-Cell Marker) Monoclonal antibody (M05470). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-07-19
Question
Does anti-Kappa Light Chain (B-Cell Marker) Monoclonal antibody M05470 work for IHC with lymph node?
B. Edwards
Verified customer
Asked: 2019-06-28
Answer
According to the expression profile of lymph node, IGKV1D-16 is highly expressed in lymph node. So, it is likely that anti-Kappa Light Chain (B-Cell Marker) Monoclonal antibody M05470 will work for IHC with lymph node.
Boster Scientific Support
Answered: 2019-06-28
Question
We are currently using anti-Kappa Light Chain (B-Cell Marker) Monoclonal antibody M05470 for human tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on goat tissues as well?
S. Singh
Verified customer
Asked: 2015-01-20
Answer
The anti-Kappa Light Chain (B-Cell Marker) Monoclonal antibody (M05470) has not been validated for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-01-20