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Product Info Summary
| SKU: | PA1630 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Kallikrein 5/KLK5 Antibody Picoband®
View all Kallikrein 5 Antibodies
SKU/Catalog Number
PA1630
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Kallikrein 5/KLK5 Antibody catalog # PA1630. Tested in IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Kallikrein 5/KLK5 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1630)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of mouse Kallikrein 5, different from the related rat sequence by three amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1630 is reactive to Klk5 in Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
29 kDa
Calculated molecular weight
31908 MW
Background of Kallikrein 5
KLK5 (Kallikrein-related peptidase 5), formerly known as stratum corneum tryptic enzyme (SCTE) or KLKL2, is a serine protease expressed in the epidermis. The KLK5 gene is one of the fifteen kallikrein subfamily members which is located on 19q13.41. KLK5 has been suggested to regulate cell shedding in conjunction with KLK7 and KLK14, given its ability to degrade proteins which form the extracellular component of cell junctions in the stratum corneum. The KLK5 gene contains 5 coding exons spanning 9.3. KLK5 expression is upregulated in a breast cancer cell line in the presence of estrogens and progestins and is associated with more aggressive forms of epithelial ovarian carcinoma.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1630 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Rat, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Mouse
Positive Control
WB: Mouse Liver Tissue
IHC: Rat Testis Tissue
Validation Images & Assay Conditions
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Anti-Kallikrein 5 antibody, PA1630, Western blotting
WB: Mouse Liver Tissue Lysate
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Anti-Kallikrein 5 antibody, PA1630, IHC(P)
IHC(P): Rat Testis Tissue
Protein Target Info & Infographic
Gene/Protein Information For Klk5 (Source: Uniprot.org, NCBI)
Gene Name
Klk5
Full Name
Kallikrein-5
Weight
31908 MW
Superfamily
peptidase S1 family
Alternative Names
Kallikrein 1-related peptidase b5 ;Kallikrein related-peptidase 5 ;Klk5 ; KLK5 KLK-L2, KLKL2, SCTE kallikrein related peptidase 5 kallikrein-5|kallikrein-like protein 2|stratum corneum tryptic enzyme
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Klk5, check out the Klk5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Klk5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Kallikrein 5/KLK5 Antibody Picoband® (PA1630)
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No publications found for PA1630
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1 Customer Q&As for Anti-Kallikrein 5/KLK5 Antibody Picoband®
Question
We are currently using anti-Kallikrein 5/KLK5 antibody PA1630 for mouse tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says mouse, rat. Is it likely that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2020-03-17
Answer
The anti-Kallikrein 5/KLK5 antibody (PA1630) has not been validated for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-17
