Kallikrein-5 Antibodies

Kallikrein-5 (KLK5)

May be involved in desquamation. .

Gene Information

Human
Gene Name:	KLK5
Uniprot:	Q9Y337
Entrez:	25818

Family

Belongs to:
peptidase S1 family

Alternative Names

EC 3.4.21; EC 3.4.21.4; Kallikrein 5; Kallikrein-like protein 2; kallikrein-related peptidase 5; KLK5; KLKL2; KLK-L2; KLK-L2EC 3.4.21.-; SCTE; SCTEkallikrein-5; Stratum corneum tryptic enzyme

Molecular Weight

Mass (kDA):
32.02 kDA

Genomic Context

Human
Location:	19q13.41
Sequence:	19; NC_000019.10 (50943299..50953038, complement)

Tissue Specificity

Expressed in skin, breast, brain and testis. Expressed at the stratum granulosum of palmar skin.

Subcellular Localizations

Secreted.

Diseases

• Malignant Neoplasms
• Neoplasms
• Malignant Neoplasm Of Ovary
• Dermatologic Disorders
• Ovarian Neoplasm
• Malignant Neoplasm Of Breast
• Dermatitis
• Carcinoma
• Dermatitis, Atopic
• Malignant Paraganglionic Neoplasm
• Malignant Neoplasm Of Prostate
• Ichthyosis Linearis Circumflexa
• Mammary Neoplasms

• Inflammation
• Prostatic Neoplasms
• Cell Invasion
• Tissue Adhesions
• Psoriasis
• Rosacea
• Skin Diseases, Genetic

Interacting Proteins

• FLG

Pathways

• Pathogenesis
• Localization
• Proteolysis
• Keratinocyte Differentiation
• Reverse Transcription
• Angiogenesis
• Keratinization
• Immune Response
• Secretion
• Cell Adhesion
• Gene Silencing
• Skin Development
• Pigmentation

• Cell Cycle Arrest
• Embryo Implantation
• Cell Cycle
• Innate Immune Response
• Cell-cell Adhesion
• Histone Deacetylation
• Cell Proliferation

PTMs

• Cleavage
• Reduction
• Glycosylation

• Deacetylation
• Methylation
• Deglycosylation

Research Areas

• Cancer

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/KLK5

Boster Bio: Best Uses Of The KLK5 Marker

If you're a biomedical researcher, then you've likely read articles on how you can optimize your research by using Boster Bio's primary antibody. These antibodies have been validated using Western Blotting and ELISA. You can also learn how to improve your research by using Boster Bio's optimization guidelines. Troubleshooting guides can help you avoid potential mistakes that could lead to your experiment to fail.

Boster Bio's research community trusts Boster Bio's primary antibody.

Boster Bio was founded in 1993 by Steven Xia and is a leader in high-specificity, antigens with high sensitivity for life sciences research. The company has invested two decades into perfecting its techniques and technology to ensure the highest quality products. Boster Bio products are cited in more than 29,000 publications. The antibodies are routinely tested to be used with ELISA, WB and Flow Cytometry as well as IHC.

Boster Bio's primary antibodies are validated using Western Blotting

Boster Bio makes high-quality primary antibodies as well as ELISA kits. Every product is thoroughly tested and approved for Western blotting or ELISA. Boster's quality guarantee guarantees that primary antibodies work exactly as they say. It also offers a free secondary antibody when you buy a primary antibody from the company. Boster Bio offers antibodies that can be used in human and mouse samples.

It is vital to evaluate the performance of any antibody within the context of the test that is being used to verify its validity. Primary antibody performance can be sensitive to minor variations in assay conditions, so an accurate analysis of these antibodies is vital to get reproducible results. Performing an assay-specific validation of an antibody will ensure that it performs as expected in the assay. The antibody's specificity and its selective binding to a protein target will be tested for reproducibility.

Collaboration between vendors, users and publishers is crucial to ensure the high-quality validation of antibodies. The user must perform well-designed tests to confirm the effectiveness of the antibodies. The supplier should provide top quality antibodies and a complete details of the validation procedures. The publisher should also establish guidelines for the validation of antibodies and make them enforced. Working together, researchers can achieve reproducibility. All parties who are involved in the process of validation of antibodies can benefit from this collaboration.

Boster Bio's primary antibody is validated by Western Blotting using a unique technique that employs a dual labeling technique. This unique technique allows researchers to identify proteins of interest , without the use of secondary antibodies. In addition to the increased specificity, primary antibodies also provide superior results in proteomics, histopathology, and immunohistochemistry. Flow Cytometry can be used to measure the number of certain types of cells.

To be effective in pan/PTM analysis, primary antibodies must be validated using a specific assay. PTM-directed antibodies must be specific to the modification of the protein targeted. Cross-reactive bands can lead to problems in the interpretation of data and analysis. Therefore, the primary antibody should be validated with separate Western Blottings, and then tested using secondary antibodies that are mismatched to determine potential cross-reactivity.

Boster Bio's primary antibodies have been verified by ELISA

In addition to producing high-quality antibodies, Boster Bio also produces high-quality ELISA kits. These kits are formulated to detect biomarkers that are used in developmental neuroscience, biology as well as inflammation and cancer. Boster Bio's products are validated on ELISA and IHC. All reagents are accessible at tebu-bio, the company's online store. Customers interested in purchasing reagents can contact the company to get assistance.

The process of validating antibodies involves examining their specificity, reproducibility and phospho-specificity. The antibodies are additionally tested against paraffin-embedded pellets, cell lines treated with siRNA tissue sections, FFPE tissue, and cells that have been treated with siRNA. To ensure that the best results, boster bio tests their antibodies against cell lines that express known targets and cells expressing targeted expression that is altered by those targets.

The validation process is a crucial step in the creation of any antibody and Boster Bio's primary antibody are validated by ELISA. Boster Bio's primary antibody are validated by ELISA to make sure that the reagent performs effectively. ELISA tests are conducted by expert immunologists who are familiar with the methodology and ensure that the antibodies are as accurate as possible.

Companies 2-5 displayed moderate levels of the validation of antibodies. They did not provide detailed descriptions of their validation process. The datasheets included background information about the immunogen and the target. Three of the four companies offered the complete sequence, while the fourth identified the region around the site of phosphorylation. Companies 2 and 3 provided recommended applications and starting dilutions for their products. Most provided examples of antibodies successful in identifying targets in the recommended applications.

ELISA is an enzyme-immunisation assay that employs the most efficient plate-coating conditions. Microplates must be coated with at least 400 ng/cm2 of protein. To ensure that the results are reliable microplates should have low CV (CV value). The color of the plate varies based on the type of signal and is either transparent polystyrene flat bottomed plate or white opaque plates.

For sandwich ELISAs, the primary antibodies must recognize different, non-overlapping epitopes. A matched pair consists of antibodies that recognize targets from various sources. A matched pair permits an matched pair to be employed in sandwich ELISA. The matched pairs must have specificity to the primary antibody. For instance an antibody for ER a (clone 1D5) from Dako in Glostrup, Denmark, is highly immunogenic and nuclear.