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Product Info Summary
| SKU: | PA1626 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Kallikrein 2/KLK2 Antibody Picoband®
View all Kallikrein 2 Antibodies
SKU/Catalog Number
PA1626
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Kallikrein 2/KLK2 Antibody catalog # PA1626. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Kallikrein 2/KLK2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1626)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Kallikrein 2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1626 is reactive to KLK2 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
29 kDa
Calculated molecular weight
28671 MW
Background of Kallikrein 2
KLK2 (KALLIKREIN 2), also called GLANDULAR or PROSTATIC, is a protein that in humans is encoded by the KLK2 gene, and is particularly associated with prostatic tissue. The KLK2 is a member of glandular kallikrein gene family that comprises 25 to 30 highly homologous genes that encode specific proteases involved in the processing of biologically active peptides. The KLK2 gene is mapped to 19q13.33. And the KLK2 gene contains 5 exons. An alternative KLK2 transcript, which they call KLK2-linked molecule (KLM), that arises from the use of an alternate donor site within intron 1. KLM shares only the N-terminal 15-amino acid signal peptide with the original KLK2 protein; the mature proteins display no similarity.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1626 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: human PANC whole cell, human Hela whole cell, human 293T whole cell
IHC: human prostatic cancer tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Kallikrein 2 using anti-Kallikrein 2 antibody (PA1626).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 40 ug of sample under reducing conditions.
Lane 1: human PANC whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human 293T whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Kallikrein 2 antigen affinity purified polyclonal antibody (Catalog # PA1626) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Kallikrein 2 at approximately 29 kDa. The expected band size for Kallikrein 2 is at 29 kDa.
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Figure 2. IHC analysis of Kallikrein 2 using anti-Kallikrein 2 antibody (PA1626).
Kallikrein 2 was detected in a paraffin-embedded section of human prostatic cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Kallikrein 2 Antibody (PA1626) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For KLK2 (Source: Uniprot.org, NCBI)
Gene Name
KLK2
Full Name
Kallikrein-2
Weight
28671 MW
Superfamily
peptidase S1 family
Alternative Names
Kallikrein-2;3.4.21.35;Glandular kallikrein-1;hGK-1;Tissue kallikrein-2;KLK2; KLK2 KLK2A2, hGK-1, hK2 kallikrein related peptidase 2 kallikrein-2|glandular kallikrein 2|glandular kallikrein-1|kallikrein 2, prostatic|tissue kallikrein-2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on KLK2, check out the KLK2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for KLK2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Kallikrein 2/KLK2 Antibody Picoband® (PA1626)
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1 Customer Q&As for Anti-Kallikrein 2/KLK2 Antibody Picoband®
Question
We are currently using anti-Kallikrein 2/KLK2 antibody PA1626 for human tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2019-06-18
Answer
The anti-Kallikrein 2/KLK2 antibody (PA1626) has not been tested for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-06-18
