Product Info Summary
SKU: | A06684 |
---|---|
Size: | 100 µl |
Reactive Species: | Human |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-KAL1 ANOS1 Antibody
SKU/Catalog Number
A06684
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-KAL1 ANOS1 Antibody (Catalog # A06684). Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-KAL1 ANOS1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06684)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human KAL1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A06684 is reactive to ANOS1 in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 100 µL
Calculated molecular weight
76112 MW
Background of KAL1
Has a dual branch-promoting and guidance activity, which may play an important role in the patterning of mitral and tufted cell collaterals to the olfactory cortex By similarity. Chemoattractant for fetal olfactory epithelial cells.
Legouis R., Cell 67:423-435(1991).
del Castillo I., Nat. Genet. 2:305-310(1992).
Franco B., Nature 353:529-536(1991).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06684 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of extracts from HuvEc cells, using KAL1 antibody A06684.
Protein Target Info & Infographic
Gene/Protein Information For ANOS1 (Source: Uniprot.org, NCBI)
Gene Name
ANOS1
Full Name
Anosmin-1
Weight
76112 MW
Alternative Names
Anosmin-1 ;Adhesion molecule-like X-linked;Kallmann syndrome protein ;ANOS1 ;ADMLX, KAL, KAL1, KALIG1; ANOS1 ADMLX, HH1, HHA, KAL, KAL1, KALIG-1, KMS, WFDC19 anosmin 1 anosmin-1|Kallmann syndrome interval gene 1|Kallmann syndrome-1 sequence (anosmin-1)|WAP four-disulfide core domain 19|adhesion molecule-like X-linked|kallmann syndrome protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ANOS1, check out the ANOS1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ANOS1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-KAL1 ANOS1 Antibody (A06684)
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No publications found for A06684
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Customer Q&As
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6 Customer Q&As for Anti-KAL1 ANOS1 Antibody
Question
Would anti-KAL1 antibody A06684 work for WB with spinal cord?
Verified Customer
Verified customer
Asked: 2020-03-23
Answer
According to the expression profile of spinal cord, ANOS1 is highly expressed in spinal cord. So, it is likely that anti-KAL1 antibody A06684 will work for WB with spinal cord.
Boster Scientific Support
Answered: 2020-03-23
Question
Is a blocking peptide available for product anti-KAL1 antibody (A06684)?
Verified Customer
Verified customer
Asked: 2019-08-30
Answer
We do provide the blocking peptide for product anti-KAL1 antibody (A06684). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-08-30
Question
I have a question about product A06684, anti-KAL1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-06-14
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A06684 anti-KAL1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-06-14
Question
I was wanting to use your anti-KAL1 antibody for WB for human spinal cord on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human spinal cord identification?
M. Collins
Verified customer
Asked: 2018-08-28
Answer
You can see on the product datasheet, A06684 anti-KAL1 antibody has been tested for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human spinal cord in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-08-28
Question
I see that the anti-KAL1 antibody A06684 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-04-06
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-04-06
Question
We are currently using anti-KAL1 antibody A06684 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on primate tissues as well?
Z. Anderson
Verified customer
Asked: 2014-08-11
Answer
The anti-KAL1 antibody (A06684) has not been tested for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-08-11