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Product Info Summary
| SKU: | M07781 |
|---|---|
| Size: | 50 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, WB |
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Product info
Product Name
Anti-RPL14 Antibody (Center)
SKU/Catalog Number
M07781
Size
50 µl
Description
Boster Bio Anti-RPL14 Antibody (Center) (Catalog # M07781). Tested in WB, Flow Cytometry, IF application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-RPL14 Antibody (Center) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M07781)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This RPL14 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 117-147 amino acids from the Central region of human RPL14.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M07781 is reactive to RPL14 in Human
Reconstitution
Calculated molecular weight
23432 Da
Background of RPL14
Binds specifically to the 3'-terminal U-tract of U6 snRNA. May be involved in pre-mRNA splicing.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M07781 is guaranteed for Flow Cytometry, IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IF: 1:25
WB: 1:2000
FC: 1:25
Validation Images & Assay Conditions
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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U-2 OS (human osteosarcoma cell line) cells labeling RPL14 with M07781 at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on U-2 OS cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin at 1/100 dilution (red).The nuclear counter stain is DAPI (blue).
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All lanes : Anti-RPL14 Antibody (Center) at 1:2000 dilution
Lane 1: Hela whole cell lysate
Lane 2: Jurkat whole cell lysate
Lane 3: MCF-7 whole cell lysate
Lane 4: 293T/17 whole cell lysate
Lane 5: U-2OS whole cell lysate
Lane 6: A431 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 23 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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Overlay histogram showing U-2OS cells stained with M07781 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (M07781, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1g/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Protein Target Info & Infographic
Gene/Protein Information For RPL14 (Source: Uniprot.org, NCBI)
Gene Name
RPL14
Full Name
60S ribosomal protein L14
Weight
23432 Da
Superfamily
eukaryotic ribosomal protein eL14 family
Alternative Names
60S ribosomal protein L14, CAG-ISL 7, RPL14 RPL14 CAG-ISL-7, CTG-B33, L14, RL14, hRL14 ribosomal protein L14 60S ribosomal protein L14|large ribosomal subunit protein eL14
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RPL14, check out the RPL14 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RPL14: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-RPL14 Antibody (Center) (M07781)
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No publications found for M07781
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